Elementary Study On Rapid Propagation System Of Euonymus Bungeanus Maxim

Euonymus bungeanus Maxim has a graceful appearance,and has a strong plant dust-retention ability.It is a precious landscaping plant and has great value in industry and medicine.Traditional propagation methods cannot guarantee the yield and quality of seedlings due to factors such as complex seed pretreatment,limited seed resources,and slow propagation speed.It hinders the further development and utilization of the germplasm resources of E.bungeanus Maxim.The stems and callus of E.bungeanus Maxim are the test materials of this experiment,Preliminary establishment of a tissue culture and rapid propagation system of E.bungeanus Maxim to obtain high-quality seedlings and provide a theoretical basis for future large-scale industrial production.The result is as follows:1.The stem were collected in April,the infection rate was 1.3%,and the survival rate was 67.35%;The way to sterilize the stems is to first rinse with running water for1-2 hours,use 75%alcohol to disinfect the surface for 20 seconds,rinse with sterile water for 5 times,and then disinfect with 0.1%Hg Cl₂ for 7 minutes.The highest survival rate is 54.88%;2.The medium for E.bungeanus Maxim stem segments was WPM+6-BA 1.0mg/L+NAA 0.2 mg/L,the induction rate was 87.21%;The proliferation medium for E.bungeanus Maxim stem segments was 1/4WPM+6-BA 2.0 mg/L+NAA 0.1 mg/L,and the proliferation coefficient reaches 4.98;3.The medium for E.bungeanus Maxim callus induction was MS+6-BA 1.0mg/L+NAA 0.3 mg/L,and the induction rate was 87.50%;The medium for E.bungeanus Maxim callus proliferation was MS+6-BA 2.0 mg/L+2,4-D 0.4 mg/L,and the proliferation multiple reaches 2.21;The medium for E.bungeanus Maxim callus redifferentiation was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,and the induction rate was94.26%;4.The rooting medium for E.bungeanus Maxim was 1/2MS+IBA 0.2 mg/L,and the rooting rate was 78.14%.