Identification And Expression Of CAMTA Genes And Its Response To Acid-base Stress In Panax Ginseng C.A.Meyer

Ginseng(Panax ginseng C.A.Meyer)is a perennial medicinal plant in the genus Panax(Araliacede).Ginseng has a long history of application and has existed on the earth for millions of years.It is a very precious Chinese herbal medicine that has been used today.Because ginseng is susceptible to environmental pressure,many genes related to ginsenoside synthesis are affected by various environmental conditions,including extreme temperature,acid-base,and drought.Under these abiotic stresses,plants will respond and adapt through a series of p Hysiological and biochemical changes.Calmodulin-binding transcription activators(CAMTA)family of calmodulin-binding transcription activators represents the newest Ca M interacting proteins in plants,responding to a series of environmental stresses(such as drought,acid-base,cold stress,and injury).In order to initially explore the function of the CAMTA gene in ginseng,this study is based on the Jilin ginseng transcriptome database and expression database that have been established in the laboratory.The CAMTA transcription factor gene family in ginseng was identified,function prediction,p Hylogeny,expression pattern and The systematic analysis of the network has unearthed 4 genes PgCAMTA09-4,PgCAMTA11-24,PgCAMTA12-23 and PgCAMTA13-20 significantly related to ginsenoside content.The adventitious roots of ginseng were treated with acid-base stress(PH: 3.5-8.0)for 5 days,and the growth and saponins content of ginseng adventitious roots were determined;four candidate genes related to ginsenoside biosynthesis were analyzed by fluorescence quantitative analysis,and the following results were obtained :1.A total of 120 PgCAMTAs transcripts under 14 gene IDs were obtained from the ginseng transcriptome database,of which 66 PgCAMTAs had a complete open reading frame;2.Perform GO function annotation and enrichment analysis on PgCAMTAs.The results show that CAMTA protein is involved in plant biological processes,molecular functions and cellular components,and mainly concentrated in 7 functional categories at the level2 level.3.The results of the study on the expression activity of PgCAMTAs show that: the expression patterns of PgCAMTAs in the 4-year roots of 42 farm varieties of ginseng,14 tissues and organs of 4-year ginseng and 4 different annual ginseng roots showed that: The expression of PgCAMTAs has certain tissue specificity and temporal and spatial specificity,and the expression of PgCAMTAs is different in different tissues and organs,different years and different farm varieties.The expression of PgCAMTAs in the life activities of ginseng influences each other,forming a complex expression network,which is not independent of each other.This expression further affects the growth and development of plants.4.The correlation analysis between PgCAMTAs gene expression and ginsenoside content,network analysis with the cloned ginsenoside key enzyme genes,and finally predicted and obtained candidate PgCAMTAs significantly related to the synthesis of saponins,namely:PgCAMTA09-4,PgCAMTA11-24,PgCAMTA12-23 and PgCAMTA13-20.5.Acid-base stress ginseng adventitious roots,selected 4 different subfamily PgCAMTAs genes for fluorescence quantitative analysis,and found that acid-base stress treatment has effects on PgCAMTA11-24(2.02--16.53 times of untreated plants),PgCAMTA12-23(untreated 2.02-16.53 times of plants),PgCAMTA13-20(5.27-10.75 times of untreated plants)have a significant upregulation effect.The results showed that PgCAMTAs family genes play an important role in the response to acid-base stress.6.Using high performance liquid chromatograp Hy(HPLC)to determine the total saponins of the adventitious roots of ginseng after acid-base stress,the total saponin content of the adventitious roots of ginseng treated by acid-base stress increased significantly,and at the same time,the fresh weight and dry weight of ginseng adventitious roots The major changes are not obvious.The above results prove that acid-base stress in the ginseng hairy root culture system promotes the synthesis of ginsenosides,and the PgCAMTAs gene family may regulate the synthesis of ginsenosides.