QTL Mapping Of 14 Traits In F₂ Generation Of Sorghum × Sudangrass Hybrids

In this experiment,a F₂ generation population of sorghum×sudangrass hybrids was constructed and traits of 424 individuals in the F₂ generation population were measured.Genetic analysis of population 16 traits was performed using main gene-multigene analysis and single generation analysis methods.Among them,14 traits were studied for QTL mapping.First,the quantitative genetic analysis results are as follows:1.The stem diameter is in accordance with Model A＿1 and is subject to 1 pair.An additive-dominant genetic model controlled by the main gene,the major gene heritability was 30.01%.2.The optimal genetic model of plant height and leaf width is Model A＿4,which is a negative full dominant genetic model.It is inherited by a pair of major genes,and the generalized heritability is 41.01%and 45.37%,respectively.3.The optimal models of flowering stage,number of leaves,flag leaf length,ear weight,100-grain weight,shelling rate,crude protein and crude fiber are Model B＿1,which belongs to the additive-dominant-epitope of two pairs of major genes.In the mixed genetic model,the genetic rates of the main genes were 62.25%,53.1%,64.3%,66.81%,40.37%,89.11%,38.59%and 42.83%,respectively.4.The optimal genetic model for acid detergent fibers was Model B＿5,which was 2 pairs of masters.The complete dominant model of genetic inheritance,the heritability of the main gene is 59.95%.5.The length of the stem and the average length of the stem are in accordance with Model B＿6,which is an isogenic genetic model of two pairs of major genes.The main gene heritability is 49.19%and 53.44%.Second,in the QTL study of 14 traits in this population,a total of 58 QTL loci with 14traits were detected on 10 sorghum linkage groups,with a total length of 1518.8 c M and an average distance between markers of 26.19 c M;the marker interval ranged from 0.3 c M.-47.7c M range.conclusion as below:1.In the phenotypic traits,three QTL loci were detected in the flowering stage,which were located on chromosomes 3,7,and 10,respectively;five QTL loci were detected on plant height,which were located on chromosomes 5,6,7,8,and 10;three QTL loci were detected in the number of leaves,which were located on chromosomes 1,3 and 10,respectively;six QTL loci were detected on the stem length,which were located on chromosomes 1,2,5,7,8,and 10,respectively.four QTL loci were detected on the flag leaf length,which were located on chromosomes 2,3,9,and 10,respectively;seven QTL loci were detected on leaf width,which were located on chromosomes 1,2,3,4,7,9,and 10,respectively;four QTL loci were detected on the average stem length,which were located on chromosomes 4,5,8,and 10,respectively;five QTL loci were detected in stem stems,which were located on chromosomes 1,3,7,9,and10,respectively.2.In the yield traits,five QTL loci were detected on the ear weight,which were located on chromosomes 3,4,7,9,and 10,respectively;four QTL loci were detected on 100-grain weight,which were located on chromosomes 2,3,4,and 7,respectively;five QTL loci were detected in the shell rate,which were located on chromosomes 2,3,4,7,and 8,respectively.3.In the feed quality traits,one QTL locus was detected in the crude protein,which was located on chromosome 4.a QTL locus was detected on the crude fiber,which was located on chromosome 6.acid detergent fibers detected five QTL loci on chromosomes 3,4,6,7,and 10,respectively.In the QTL mapping study,four QTL loci were located on chromosomes 2,3,9,and 10;seven QTL loci were detected on leaf width,located on chromosomes 1,2,3,4,7,9,and 10;Four QTL loci were detected on the chromosomes,which were located on chromosomes 4,5,8,and 10;five QTL loci were detected on stems,which were located on chromosomes 1,3,7,9,and 10;Five QTL loci were detected on chromosomes 3,4,7,9,and 10;four QTL loci were detected on 100,4,4,7,and chromosomes;Five QTL loci were detected on chromosomes 2,3,4,7,and 8 respectively;one QTL locus was detected on the chromosome 4 on the crude protein;one QTL locus was detected in the crude fiber.Located on chromosome 6;Washing the fiber 5is detected QTL loci respectively 3,4,6,7,10 located on chromosome number.The flag leaf length contributed the largest contribution rate in the sam36933-sam35926 marker region of chromosome 3,which was 11%.The crude fiber and acid detergent fiber were labeled to the Xtxp265-Xcup12 and Xcup12-sam79056a regions of chromosome 6,respectively,with a contribution rate of 10.7.%and 12.6%;flowering stage,leaf number,leaf width,stem diameter and ear weight all reached the highest contribution rate in the sam 50778-sam48932 marker interval of chromosome 10,and the contribution rates were 19.4%,14.2%,14.9%,respectively.11.9%,12.8%,there are multiple major gene loci in this interval;for areas with higher QTL mapping of traits,primers can be encrypted in this interval,and the positions of each trait are marked in In a smaller interval,lay the foundation for the next step of fine positioning.