Study On The Relationship Between Vitamin D Receptor Gene And Osteoporosis In Dai People In Yunnan Area

Objective To study the genotype and allele distribution frequency of vitamin D receptor(VDR)gene in Dai people in Yunnan,and to explore the correlation between VDR gene Fok I and Bsm I polymorphism and Osteoporosis(OP)in Dai people in Yunnan.The genetic polymorphism was compared with that of the Han population in Yunnan.To explore the genetic susceptibility factors of osteoporosis by screening high-risk groups.Method A case-control study was conducted.A total of 398 patients with osteoporosis and hypobone mass(268 Dai and 130 Han)and 245 healthy subjects(127 Dai and 118 Han)in Yunnan were selected as the study subjects.All patients with osteoporosis and hypobone mass met the diagnostic criteria recommended by WHO in 1994.Clinical data including height,weight and waist circumference were collected,and Triglyceride(TG)and High density lipoprotein(HDL)cholesterol were measured.HDL-C),Low Density Lipoprotein Cholesterol(LDL-C)and other biochemical indicators.Polymerase chain reaction-restriction fragment length polymorphism was used in the study of Restriction fragment length.PCR-RFLP)technique was used to determine the polymorphism of VDR gene Fok I and Bsm I.BMD of lumbar L1-I4 was measured using Dual Energy X-Ray Ababsorptiometry(DEXA)to compare the relationship between the genotypes and allele frequency distribution of the two loci and osteoporosis.The correlative factors of osteoporosis were analyzed.Results(1)The genotype and allele frequency distribution of VDR gene Fok I and Bsm I locus in Dai and Han populations in Yunnan region were analyzed by Hardy-Weinberg equilibrium.Hwe P>0.05 was in line with Hardy-Weinberg equilibrium.The selected population was in line with Mendelian genetic rule and was representative of the population.(2)There was no significant difference in the distribution frequency of VDR gene Fok I and Bsm I genotypes and alleles between Dai population and Han population(P>0.05).The subjects were divided into three groups according to the diagnostic criteria.There were significant differences in allele frequency between osteoporosis group and osteopenia group(P<0.05).There were no significant differences in genotype and allele frequency between osteoporosis group and osteopenia group(P>0.05).Male under 50 years old and premenopausal women were divided into two groups according to Z value.There was no significant difference in genotype and allele frequency distribution of two loci in low bone mass group compared with control group(P>0.05).There was no significant difference in the distribution of genotype and allele frequency of the two loci between Dai and Han groups(P>0.05)after stratification by ethnic groups among 3 T-value groups and 2 Z-value groups.(3)Compared with the control group,there were statistically significant differences in age,height,weight,Body Mass Index(BMI),waist circumference,HDL-C,LDL-C and sex composition in the case group(osteoporosis group and osteopenia group)in Yunnan region(P<0.05).Stratified by ethnic group,the age and HDL-C of the Dai and Han groups were higher than those of the control group,while the body weight and BMI were lower than those of the control group,with statistical significance(P<0.05).Secondly,there were significant differences in height,waist circumference and gender between the Dai case group and the control group(P<0.05).There was no significant difference between the other indexes and the control group(P>0.05).(4)The results of linear correlation analysis showed that T value of men aged 50 and above and postmenopausal women was positively correlated with height,weight,BMI and waist circumference(P<0.05),and negatively correlated with age,HDL-C and LDL-C(P<0.05),but had no correlation with TG(P>0.05).The Z-values of men aged 50 and younger and premenopausal women were positively correlated with height and negatively correlated with age(P<0.05),but not with body weight,BMI,waist circumference,HDL-C,LDL-C and TG(P>0.05).(5)Multivariate binary Logistic regression analysis showed that age increase and female were the risk factors for osteoporosis in Yunnan and Dai population(P<0.05);The decrease of HDL-C level was a protective factor for osteoporosis in the whole subjects and Dai population(P<0.05).The increase of age and LDL-C level were the risk factors for osteoporosis in Han population(P<0.05).Conclusion(1)There was no difference in the genotype and allele frequency distribution of Fok I and Bsm I of VDR genes between Dai and Han populations in Yunnan.(2)There was no correlation between VDR gene Fok I and Bsm I abnormal loci polymorphism and the occurrence of osteoporosis.(3)Age,body weight,BMI and HDL-C were associated with the incidence of osteoporosis in Yunnan.Age,height,weight,BMI,waist circumference and HDL-C were correlated with T value,and height was correlated with Z value,suggesting that the incidence of osteoporosis in the elderly was more closely related to risk factors than that in the young.(4)Age,women,and increased LDL-C level are the risk factors for osteoporosis in Yunnan population,while decreased HDL-C level is the protective factor for osteoporosis.