Association Between Bone Mineral Density And Genetic Polymorphisms Of Vitamin D Receptor Gene,calcitonin Receptor Gene In Post-menopause Han Population Of Women From Xinjiang,in China

Objective:To investigate the association of bone mineral density?BMD?with vitamin D receptor?VDR?gene and calcitonin receptor?CTR?gene polymorphisms in Han women from Xinjiang,in China;and to discuss the genetic susceptibility factors of pathogenesis of osteoporosis?OP?.Methods:VDR and CTR genotypes were defined with polymerase chain reaction-restriction fragment length polymophism?PCR-RFLP?,so as to analyze distribution of VDR and CTR polymophisms of 336Han women from Xinjiang,in China.BMD was measured by dual energy X-ray absorptiometry?DEXA?at the lumbar spine(L_2-4),Ward's triangle,great trochanter,and femoral shaft.Comparing the difference of BMD values of different locations in different genotypes.Results:336 women were included in the study.?1?The frequencies of VDR and CTR genes and alleles in the study subjects were in accordance with the Hardy-weinberg equilibrium law.Subjects were divided into two groups according to T values:osteoporosis group?90 cases?and non-bone group?246 cases?.The individual genotypes of VDR receptor gene and CTR receptor gene and their allele frequency were analyzed.The frequency distribution of VDR and CTR genes was not statistically significant?P>0.05?.?2?There was a close relationship between age and BMD values of various sites.As the age decreased,the BMD value of each site decreased,and there was a negative correlation between them?P<0.05?.BMI was closely related to BMD values of various sites.As the BMD was increased,Bone mineral density was increased in all sites and there was a positive correlation?P<0.05?.?3?The VDR-BB type?4 cases?in the osteoporosis group showed a higher degree of BMD compared with VDR-Bb?12 cases?and VDR-bb type?74 cases?,there was no significant difference between the groups?P>0.05?.There was no significant difference in the BMD values??between the CTR-CC type?73 cases?and the CTR-CT type?17 cases??P>0.05?;non-osteoporosis group VDR The BMD value of-bb type?216 cases?was higher in the 2-4,ward's triangle of the lumbar spine than in the VDR-Bb type?24 cases?,the difference was statistically significant?P<0.05?;The BMD value of VDR-bb type?216 cases?was higher in the trochanter and femoral shaft than in the VDR-Bb type?24 cases?,and the difference was statistically reduced?0.05<P<0.1?;The BMD value of the CTR-CT type?43 cases?detected in the Ward's triangle was higher than that of the CTR-CC type?203 cases?at the site,and the difference was statistically significant?P<0.05?;There was no significant difference in the BMD values of the VDR-CTR complex genotypes detected in the osteoporosis group.There was no significant difference between the composite genotypes?P>0.05?;CCBb type?19cases?was detected in the non-osteoporosis group.In the non-osteoporotic group,the BMD values of the CCBb type?19 cases?were lower than CCbb type?178 cases?,CCBB type?6 cases?,and CTBb type?5cases?CTbb type in the lumbar 2-4,ward's triangles?38 cases?,the difference was significant?P<0.05?,also lower than the other types in the large trochanter,femoral shaft,the statistical analysis of the differences have a decreasing trend?0.05<P<0.1?.Conclusion:Age and BMI was closely related to the change of BMD;Bb genotype of VDR was associated with lower BMD.Compound CCBb genotype may be regarded as a genetic marker to predict the risk of osteoporosis in Han women from Xinjiang.