To Investigate The Effect Of Platelet-rich Fibrin On The Survival Of Rabbit Ear Cartilage After Transplantation

Objective:To study the effect of autologous platelet-rich fibrin on the survival of rabbit ear cartilage after transplantation.Providing guidance for clinical applications.Methods:Sixteen New Zealand white rabbits were selected to cut and strip out ear cartilage with similar size and shape and weight of 80 mg on the symmetry of both ears.Both sides of ear cartilages were randomly divided into control group and experimental group.The piece of ear cartilage in control group was transplanted directly into the left side of the superficial fascia lacuna on the back of the rabbit,the piece of ear cartilage in experimental group combine with PRF was transplanted into the right side of superficial fascia lacuna on the back of the rabbit.General observation,weight comparison and statistical analysis and staining which includ hematoxylin-eosin staining,saffron O staining and alcian blue staining were performed after 2 weeks,4weeks,8 weeks and 12 weeks.Results:General observation and comparison of cartilage weight showed that: the weight of ear cartilage increased at 2-4 weeks,and decreased at 8-12 weeks.At each observation time,the weight of ear cartilage in the experimental group was greater than that in the control group.Statistical analysis results showed that ear cartilage weight difference between the two groups was statistically significant(Ρ < 0.05);Staining observation showed that:at each observation time,the chondrocytes in the experimental group were significantly more than those in the control group under the same microscopic scale and the same visual field.The heterochromatic cartilage matrix and blue glycosaminoglycan were more obvious in the experimental group.Conclusion:Autologous platelet-rich fibrin can promote the survival of rabbit ear cartilage after transplantation.Providing guidance for the clinical application of PRF in rhinoplasty.