| Objective:This experiment is to observe the expression of OPG / RANKL / RANK signaling pathway related factors in periapical tissues of type II diabetic mice during the occlusal movement of molars after tooth extraction,and explore its movement mechanism,so as to provide basic theoretical reference for the repair of dentition defect in diabetic patients.Methods:A total of 120 male C57 BL/6J mice with body weight between 20 and 22 g were randomly divided into three groups: diabetes group(40 mice),blank control group(40mice)and negative control group(40 mice).After adaptive feeding for 2 weeks,mice in the diabetes group were fasted for 10 h and could drink water freely during this period.Diabetic mice were induced by intraperitoneal injection of streptozotocin(STZ).Negative control group mice were intraperitoneally injected with the same amount of sodium citrate buffer as diabetic group as positive control group,one group was not treated as blank control group.Methods: The diabetic mouse model was established by intraperitoneal injection of 70 mg/kg of STZ powder with sodium citrate buffer solution(0.1 mol/ L,p H=4.2)for 5 days.After the successful establishment of diabetic mouse model,30 mice were randomly selected from each group,three molars of the right maxillary of each group were extracted,and the mice were sacrificed at 0 d,3 d,6 d,9 d and 12 d,respectively.Six mice in each group were sacrificed at each time,and the right mandibular bone of the mice was taken.The gene expression levels of OPG,RANKL and RANK in periapical tissues were determined by real-time fluorescence quantitative PCR.The protein expression levels of OPG,RANKL and RANK in periapical tissues were determined by immunohistochemistry.Results:1.The expression levels of OPG,RANKL and RANK genes and proteins in the periapical tissues of the right mandibular bone were different in the diabetic group,blank control group and negative control group from 0 to 12 days after the extraction of the right maxillary molar.2.Comparison between groups showed that: The expression of OPG gene and protein in periapical tissue of diabetic mice at 3,6,9 and 12 d was significantly lower than that of blank control group and negative control group(P<0.05),and there was no significant difference between blank control group and negative control group at 3,6,9 and 12 d(P>0.05).There was no significant difference in the expression of OPG gene and protein among the three groups at day 0(P BBB 0 0.05).Intra-group comparison showed that the expression of OPG gene in periapical tissues of mice in 3 groups decreased continuously with the extension of tooth extraction time.3.Comparison between groups showed that: The expression of RANKL,RANK gene and protein in periapical tissue of diabetic mice at 3,6,9 and 12 d were significantly higher than those in blank control group and negative control group(P<0.05),and there were no significant differences between blank control group and negative control group at3,6,9 and 12 d(P>0.05).There were no significant differences in RANKL,RANK gene,protein gene and protein expression among 3 groups at 0 d(P>0.05).The intra-group comparison showed that the expression levels of RANKL,RANK gene and protein in periapical tissues of mice in the three groups increased with the prolonging of tooth extraction time.Conclusion:The expression of OPG in periapical tissues of diabetic mice was lower than that of the corresponding control group from 0 d to 12 d,while RANKL and rank were significantly higher than those of the corresponding control group,suggesting that diabetes may further down regulate the expression of OPG in periapical tissues and up regulate the expression of OPG in the process of occlusal elongation The expression of RANKL and rank increased the ratio of RANKL / OPG,aggravated bone resorption and inhibited bone formation. |
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