Aloe Polysaccharide On Rat Ⅱ Deep Degree Burn Wound Healing Effect Research

Objective: To observe the healing effect of aloe polysaccharide on deep second degree burn wounds in rats,by measuring EGF（epidermal growth factor）,b FGF（basic fibroblast growth factor）,inflammatory mediator（TNF-α,IL-1,The expression of IL-10）and immune factors（Ig A,Ig G,Ig M）explored the mechanism of aloe polysaccharides in promoting burn wound healing,and provided scientific experimental basis for the development and application of aloe gel.Methods:150 SD rats were randomly divided into 5 groups: negative control group,positive control group,and drug-administered group 3 groups,30 rats in each group,fed in cages,freely ingested drinking water,and depilated cream for hair removal.Fasting 12 hours before anesthesia,drink water.10%chloral hydrate was intraperitoneally injected into the anesthesia,and the water bath was used to cause burns.The pathological examination confirmed deep second degree burns.The negative control group was given normal saline,the positive control group was given moist scald cream,and the administration group was given aloe polysaccharides at 4mg/ml,6mg/ml and 8mg/ml respectively.The animals in each group were administered 4 times a day,and the dynamic observation was carried out.The wound healing state and healing time were recorded.The animals were sacrificed at the 2nd,6th,10 th,14th,and24 th time points and taken.HE staining was used to observe the pathological changes of wound tissue.The expression of EGF and b FGF in wound tissue was determined by immunohistochemistry.The levels of TNF-α,IL-1 and IL-10 in inflammatory mediators were detected by ELISA.Ig A,Ig G,Ig Mcontent changes.Statistical analysis using SPSS 18.0 was statistically significant with a P value of less than 0.05.Results:1.Healing rate and healing time: positive control group ≈ 8 mg/ml administration group < 6 mg/ml administration group< 4 mg/ml administration group ≈ negative control group;2.HE stain: 8 mg/ml administration group and positive In the control group,the granulation tissue,fibroblasts,and capillaries formed at a similar rate,which was significantly faster than the other three groups.3.The expression of growth factors EGF and b FGF peaked on the 6th day,then down-regulated,and administered at 8 mg/ml.Both the group and the positive control can significantly enhance the expression of EGF and b FGF.The intensity of the expression is from strong to weak:positive control group ≈ 8mg/ml administration group < 6mg/ml administration group < 4mg/ml administration group ≈ negative control Group;4.Changes in the levels of pro-inflammatory factors（TNF-α,IL-1）: 8mg/ml was directly down-regulated in the administration group and was not significantly different from the positive control group,4 mg/ml,6 mg/ml and negative.The control group peaked on the 6th day and then down-regulated.By the 14 th day,the 6mg/ml administration group was significantly lower than the negative control group,and the 4 mg/ml administration group was significantly higher than the positive control group.The difference was statistically significant（P<0.05）;5.Changes in the content of anti-inflammatory factor IL-10: 8mg/ml in the drug-administered group peaked on the 6th day,then down-regulated,no significant difference from the positive control group Different,4mg/ml,6mg/ml group reached the peak on the 10 th day,then down-regulated,there was no significant difference between the groups on the 24 th day;6.The content of immune factors（Ig A,Ig G,Ig M）:（1）There was no difference between the 8mg/ml administration group and the positive control group,and the difference was statistically significant（P<0.05）.The 4mg/ml administration group and the negative control group had no difference.Differences,（2）Ig A,Ig M content change 8mg/ml administration group was higher than the positive control group,there was a statistical difference on the 24 th day（P<0.05）,4mg/ml administration group and the negative control group no difference,6mg/ml administration group was higher than the negative control group,and the difference was statistically significant（P<0.05）.Conclusions: Aloe polysaccharide on rat deep degree burn wounds,curative effect is distinct,by Ⅱadjusting the EGF（epidermal growth factor）,b FGF（alkaline fiber cell growth factor）,immune antibody（Ig A,Ig G,Ig M）,inflammatory mediators（TNF alpha,IL-1,IL-10）expression,promote wound healing,of which 8 mg/ml to medicine group curative effect is most remarkable.