Hematopoietic Effects And Mechanisms Of SXN On Myelosuppressed Mice

Objective Radiotherapy or chemotherapy induced myelosuppression threaten the efficacy of anticancer treatments.Hematopoietic growth factors and blood transfution were used to accelerate hematopoietic recovery in patients received chemotherapy or radiation therapy.Traditional Chinese medicines has a long history in “nourish blood”,and have been widely used in cancers assistance treatment.Shengxuening tablet(SXN),a Chinese patent medicine extracted from silkworm excrement,has been used in deficiency of both qi and blood.The present study aimed to investigate the hematopoietic effects of SXN on radiation induced myelosuppressed mice,exploring the underlying mechanisms.Methods Different concentration of SXN were added into the U937,Jurkat,SGC-7901 cells.Viability of the cells was measured by CCK-8.The cell cycle and apoptosis were detected by flow cytometry.The mice were irradiated to induce myelosuppressed model.SXN was administered by gavage.The numbers of peripheral blood were counted and body weight,spleen indices were measured.The histology of femur was examined by hematoxylin and eosin staining.The levels of thrombopoietin(TPO),erythropoietin(EPO)and granulocyte-macrophage colony stimulating factor(GM-CSF)in serum were measured by ELISA.Hemopoiesis genes in liver was detected by PCR array and RTPCR.The protein levels were detected by Western blot.Results There was no significant influence of SXN on proliferation of U937,Jurkat and SGC-7901 cells.No significant influence of SXN on the cell cycle and apoptosis of U937 and SGC-7901.In myelosuppressed model,SXN significantly increased the numbers of peripheral blood cells and improve the bone marrow morphology.The decreases of body weight and spleen indices could be reversed by SXN.Furthermore,SXN increased the levels of EPO and GM-CSF in serum.SXN enhanced the m RNA and protein expression of Jak2,Stat3 in liver.Conclusion SXN was confirmed to promote the recovery of hemopoietic function in myelosuppressed model,which attributed to improving bone marrow hematopoiesis;increasing the secretion of hematopoietic growth factors;stimulating Jak2/Stat3 signaling pathway.