The Study Of Relation Of Microsatellite Instability,Expression Of Ki-67 And TopoⅡα In Colorectal Cancer And Clinicopathological Features

Objective Colorectal cancer(CRC)is considered to be the third leading cause of cancer-related death in worldwide.The treatment and 5-year survival rate of patients with CRC depend on tumor lymph node metastasis(TNM)and pathological grade.Although improved the early screening rate of CRC and the pathological grade and stage of CRC were improved,the clinical pathological characteristics of CRC could not be predicted occurrence,development and prognosis.Some studies have shown that microsatellite instability(MSI)and the expression of Ki-67 and Topo IIα in CRC are closely related to the occurrence and development of colorectal cancer.Therefore,in this study,we detected the expression of MLH1,MSH2,MSH6,PMS2(MMRPs)and Ki-67,Topo IIα in colorectal cancer,to explore the relationship with clinicopathological features of colorectal cancer.Methods1.Collection of 115 cases of colorectal cancer patients from April 2017 to April 2018 in the Department of Pathology,Pingdingshan First People’s Hospital.Collection of general data and clinicopathological data.All specimens were strictly reviewed by two senior pathologists according to WHO diagnostic criteria.2.The immunohistochemical method was used to detect the expression of MLH1,MSH2,MSH6,PMS2 and Ki-67,Topo IIα in CRC,and evaluated the patient’s MSI.3.The relationship between Ki-67,Topo-IIα,MMRPs and MSI and the clinicopathological features of CRC was analyzed.Results1.The expression of MLH1,MSH2 and PMS2 was significantly different with age,location of the disease,and degree of differentiation(P<0.05),the expression of MLH1 was also significantly different with tumor size(P<0.05);and the expression of MSH6 was significant statistical difference with the degree of differentiation(P<0.01),.2.MSI-H was significant statistical difference with age,location of the disease,tumor size and degree of differentiation(P < 0.01).3.The expression of Ki-67 and Topo IIα had no significant difference with the clinicopathological characteristics of CRC.4.The expression of Topo IIα was significantly different with the expression of MLH1 and PMS2(P<0.05).The expression of Topo IIα was significantly different with MSI-H(P<0.05).Conclusions The expression of MMRPs and MSI-H were correlated with clinicopathological features.The expression of Topo IIα were correlated with the expression of MLH1 and PMS2 and MSI-H.