Study On M1 And M2 Monocytes/macrophages In OSAHS With Non-small Cell Lung Cancer Patients

Background:Studies have shown that tumor-associated macrophages(TAMs)are closely related to malignant tumors,and the relationship between them has been reported in many literatures.Epidemiological investigation and animal and ce-11 experiments have confirmed that OSHAS is also closely related to the occurrence and development of tumors,but the specific mechanism remains unclear.Currently,there are few studies on whether OSHAs promote tumor development by affecting the function of TAMS,and the role of TAMS in patients with OSAHS complicated with lung cancer is even less studied.Objective:The changes of Ml and M2 monocytes in peripheral blood of lung cancer patients and OSAHS patients with lung cancer and the M1 and M2 TAMs in lung cancer tissues were compared to explore the role of TAM in promoting the occurrence and development of lung cancer by OSAHS.Methods:The study was divided into two parts.In the first part,the subjects were divided into three groups,including 8 healthy controls,including 3 males and 5 females.In lung cancer group,there were 15 cases,including 9 males and 6 females.There were 16 patients in OSAHS combined with lung cancer group,including 13 males and 3 females.All subjects received sleep monitoring,and 8ml of peripheral blood was collected.Peripheral blood mononuclear cells were isolated by density gradient centrifugation.M1 mononuclear cells were labeled with CD 14 and CD80,and M2 mononuclear cells were labeledwith CD 14 and CD206.In the second part,the subjects were divided into two groups.The lung cancer group had 15 patients,including 9 males and 6 females.There were 15 patients in the OSAHS group with lung cancer,including 12 males and 3 females.Lung cancer tissues were collected from the subjects through percutaneous lung puncture or surgical resection,and then paraffin sections with a thickness of 4μm were prepared.The distribution of M1 and M2 TAMs in lung cancer tissues was analyzed by immunohistochemical staining.All data were statistically analyzed using SPSS 23.0 software.Results:1.The ratio of M2/M1 mononuclear cells in peripheral blood of control group,NSCLC group and OSAHS with NSCLC group was 0.840±0.167,1.323±0.295 and 2.873±0.384,respectively,with statistically significant differences(F=9.469,P<0.01).The OSAHS group with NSCLC was higher than the NSCLC group a nd the control group,and the difference was statistically significant(P均<0.01).The NSCLC group was higher than the control group,but the difference was not statistically significant(P>0.05).2.The density of M2 TAMs in the stroma of OSAHS patients with NSCLC tended to be higher than that of NSCLC patients,but the difference was not statistically significant(P>0.05).There was a negative correlation between the density of M2 type TAMs in thestroma of cancer and the overall survival.As the density of type M2 TAMs inthe stroma of cancer increased,the prognosis tended to get worse,but the difference was not statistically significant(P>0.05).Conclusion:The results of this study suggested that the proportion of M2 monocytes in the peripheral blood circulation of OSAHS with non-small cell lung cancer patients and the infiltration of M2 type TAMs in the interstitium of lung cancer increased,suggesting that M2 type TAMs may play a role in the promotion of OSA HS in the development of NSCLC.