Study On The Correlation Between The Abnormal Sperm Parameters And The Level Of The Inflammatory Factors In Gingival Crevicular Fluid

Objective: The relationship between periodontal disease and male sperm parameters has not been determined.In this cross-sectional study,we compared the difference of important inflammatory factors in gingival crevicular fluid(GCF)and sperm between the case group and the control group to explore the possible mechanism of periodontitis affecting the men’s sperm quality through the gingival crevicular fluid micro-environment.Materials and methods: 74 males aged 21 to 48 were recruited from2018 to 2019 in the Ninth People’s hospital.The participants were divided into case and control group according to the sperm parameter,37 subjects in each group.The information of age,weight,height,personal habits,etc.was collected by questionnaire.Oral examination was carried out and periodontal index including probing depth,clinical attachment loss and bleeding index were recorded.The samples of gingival crevicular fluid and semen were collected and tested by Enzy linked immunosorbent assay.T-test,rank sum test and Spearman correlation analysis were used to compare the differences of important inflammatory factors between the two groups.Analyzing the correlation between gingival crevicular fluid inflammatory factors and abnormal sperm parameters were used to explore the impact of periodontal disease on male infertility.Results: The degree of attachment loss in the case group(1.30±0.91mm)was higher than that in the control group(1.12±0.62mm),but there was no significant difference.The levels of IFN-γ,IL-1β,IL-8,TNF-a,MMP-8,MMP-9 and PGE-2 in the gingival crevicular fluid of the case group were significantly higher than those of the control group(P < 0.01),while the levels of IL-6 and IL-10 in the gingival crevicular fluid were significantly lower than those of the control group(P < 0.01).The values of IFN-γ,IL-1 β,IL-8,TNF-a,MMP-8,MMP-9 and PGE-2 in semen of the case group were significantly higher than those of the control group(P< 0.01),while the values of IL-6 and IL-10 in semen were significantly lower than those of the control group(P < 0.01).The trend of inflammatory factors and the enzyme in gingival crevicular fluid and semen was the same,and there was a certain correlation between them.Semen parameters(semen density,total sperm count,forward movement and activity rate)were significantly correlated with the inflammatory mediators in GCF.Conclusions: The level of inflammatory factors in gingival crevicular fluid of patients with abnormal semen parameters was significantly different from that of normal group,and this difference may be earlier and more sensitive than the clinical periodontal feature.There is a correlation between periodontitis and male infertility,and it may affect each other through inflammatory factor channels.