The Relationship Between GCF MMP-8 And TIMP-1 Levels And Periodontal Disease

Objective: Periodontal disease is one of major oral diseases, which not only cause teeth loss, but also threaten human teeth. Recently, close attention was paid to the family of matrix metalloproteinases (MMPs) and its inhibitory factor which play a very important role in many physiological and pathological processes through breaking down the extracellular matrix (ECM).A large number of studies have shown that the imbalance of MMPs and TIMPs, caused by many endogenous and exogenous factors, leads to over-expression of MMPs and excessive degradation of extracellular matrix, resulting in the destruction of the tissue structure and tissue function and a variety of diseases, such as inflammation, cancer, periodontal disease, multiple sclerosis and so on.The aim of this study is to detect and compare the levels of MMP-8 and TIMP-1 in normal group, chronic marginal gingivitis and chronic periodontitis group using enzyme-linked immunosorbent assay (ELISA), furthermore, to analyze the relationship between main clinical indicators of periodontal and the levels of the MMP-8 and TIMP-1, and to explore fesability of MMP-8 as of objective indicators for periodontal tissue destruction and inflammation degree.Method:1 48 cases were selected in this study, including 22 males and 26 females, aged 22-56 years. According to the diagnostic criteria suggested in 1999 International Workshop for a Classification of Periodontal Diseases and Conditions, cases were divided into periodontal health group, the chronic marginal gingivitis group and chronic periodontitis group. The first or second molar was chosen as subjects every case, composing 48 samples.2 In this study filter paper strip was used to collect GCF. GCF was collected in the mesio-buccal site of each tooth using duplicate sampling method. Whatman No. 3 filter paper with a size of 2mm×10mm was weighed and laid in the buccal gingival sulcus each teeth. 30s later, filter paper was removed and placed in Eppendorf tube. After one minute, above procedure was repeated and another piece of filter paper was placed in the same Eppendorf tube. Finally, the tube was weighed again and placed in refrigerator with a temperature of -20℃.3 The clinical periodontal indicators were:GI (gingival index): According to the criteria proposed by L?e and Silness, GI was divided into 0-3 levels.PLI (plaque index): According to the criteria proposed by L?e and Silness, PLI was divided into 0-3 levels.PD (probing depth): The distance from the end of periodontal pocket to the gingival margin .AL (attachment loss): The distance from the end of the periodontal pocket to the enamelo-cemental junction.4 MMP-8 and TIMP-1 in GCF were tested using ELISA .Results:1 The main clinical indicators showed statistically significant difference among the periodontal health group, the chronic marginal gingivitis group and the chronic periodontitis group.2 The GCF volume of the periodontal health group, the chronic marginal gingivitis group and the chronic periodontitis group were:0.68±0.22ul,0.98±0.25ul and 1.22±0.62ul.The result of statistical analysis is P<0.05,so the difference was statistically significant. Indicating that with periodontal tissue inflammation increases, gingival sulcus the content of GCF increased.3 The average concentration of MMP-8 of periodontal healthy group, chronic marginal gingivitis group and chronic periodontitis group were 0.62±0.09ug/L,0.68±0.02ug/L and 0.78±0.05ug/L.The result of statistical analysis was P<0.05, so the difference was significant. Indicating that with periodontal tissue collected increase in inflammation, MMP-8 concentration increased.4 The average concentration of TIMP-1 of periodontal healthy group, chronic marginal gingivitis group and chronic periodontitis group were 3.55±0.93mg/L,3.42±1.49mg/L and 2.88±1.30mg/L.The result of statistical analysis was P>0.05.Indicated that TIMP-1 levels did not change significantly with periodontal tissue inflammation increases.5 It was correlated statistically in the concentration of MMP-8 and the plaque index, the gingival index, the probing depth and the attachment loss.Conclusion:1 The results shows that the amount of collected GCF increased significantly with the appearance of periodontal lesions and increasing severity, suggesting that the amount of GCF can reflect the extent of periodontal lesions so it can be considered as one of reference indicator of periodontal condition.2 Experiment shows that the concentration of MMP-8 in GCF increased significantly with the periodontal degeneration, suggesting that the concentration of MMP-8 in GCF reflects the extent of periodontal lesions, so it can be considered as one of reference indicators of evaluating the degree of periodontal degeneration.3 The results shows that there is a statistically significant in the increasing of the level of MMP-8 / TIMP-1 in GCF, so it can be considered as one of reference indicators of evaluating the degree of periodontal degeneration.4 There is a statistical correlation between the concentration of MMP-8 in GCF and the gingival index, the plaque index, the probing depth and the attachment loss. However, according to statistical principles the numerical coefficient r which determines the level of relativity was not large, so it needs a continous study.