Studies On Chemical Constituents And Bioactivities Of Marine Fungus Paraconiothyrium Sp. S212B

Marine fungus are an important part of marine microbial resources,The unique ecological environment of the ocean requires marine fungus to produce some secondary metabolites to compete for limited nutrients and defend themselves.The structure and biological activity of these secondary metabolites are different from that isolated from terrestrial fungus,and they play an important role in discovery of drug lead compounds.This paper studied the secondary metabolites and bioactivities of marine fungus Paraconiothyrium sp.S212 B based on the OSMAC strategy.We have changed the culture conditions in order to activate the silencing genes of Paraconiothyrium sp.S212 B,and obtained new and active secondary metabolites.The main contents are as follows:(1)Sixteen fermentation conditions were screened for the target strain,including 8culture medium and 8 culture medium which simulate ocean salinity.The best one was be chosen to expand the fermentation after the analysis of TLC and HPLC.(2)We used multiple chromatographic separation techniques(silica/RP-silica column,Sephadex LH-20,HPLC)and spectroscopy analysis(1D/2D-NMR,HRMS).Twelve compounds were isolated,including 1 new compound and 11 known compounds.The compounds’ structure include ten-membered macrolides,steroids,fatty acids,benzene ring derivatives,cyclic dipeptides,etc.(3)We derivatized the ten-membered macrolide compound modiolide A which has a very high yield and few research reports,obtained 15 compounds.The results of the anti-neuritis activity test showed that most derivative compounds are better than modiolide A.It provides a leading role in the subsequent study of the anti-neuritis activity of marine fungi.