Study On Improving NK Cell Activity In Leukemia Mice

Objective To observe the effects of Interleukin-2(IL-2),Interleukin-12(IL-12),Interleukin-15(IL-15),Tumor Necrosis Factor-a(TNF-a)and different combinations on the survival time of Leukemia mice;To further analyze the changes of Natural Killer cells(NK)in peripheral blood,spleen and bone marrow of Leukemia mice before and after cytokine treatment,and to preliminarily explore the effect of cytokines on the survival time of Leukemia mice The mechanism of rat survival time.Method 1.The model of Acute T-Lymphoblastic Leukemia(T-ALL)in non irradiated mice was established.The T-ALL mice were divided into 13 groups with 5mice in each group.The control group was given phosphate buffer(PBS),and the survival time of T-ALL mice was observed;2.On the basis of different concentrations of cytokines,the survival time of T-ALL mice in high concentration IL-12 group was significantly prolonged.In order to further verify the effect of high concentration of IL-12 and its combination on the survival time of T-ALL mice,T-ALL mice were divided into IL-12 group,IL-12 +IL-2 group,IL-12 + IL-15 group,IL-2 + IL-12 + IL-15 group and control group.The cytokines were high concentration,with 5 mice in each group.The survival time of T-ALL mice was observed by intraperitoneal injection;3.The survival time of T-ALL mice treated with high concentration of IL-12 was significantly prolonged.In order to explore the mechanism of prolonging the survival time of T-ALL mice and further compare the changes of NK cell number in T-ALL mice before and after cytokine treatment.The day of successful modeling of T-ALL mice was set as day 0.On day 1 and day 3,the eyeballs were removed to collect peripheral blood,and then the mice were killed by neck cutting.The spleen and bone marrow were separated to obtain mononuclear cell suspension.The number of NK cells was detected by flow cytometry after filtration,centrifugation,peripheral blood splitting and resuspension.On the fourth day after successful modeling,high concentration of IL-12 was injected intraperitoneally into T-ALL mice for 7 days,and the mice were killed on the fifth,eighth and tenth days respectively.The same method was used to detect the number of NK cells dynamically.Results 1.The survival time of T-ALL mice treated with different concentrations and cytokines: cytokines can prolong the survival time of T-ALL mice.All the T-ALL mice in the high concentration IL-12 group survived for more than 18 months;one T-ALL mouse in the high concentration IL-15 group survived for more than 18months;the median survival time of the control group was 1 month.2.The survival time of T-ALL mice in high concentration IL-12 group and combination group: the survival time of T-ALL mice in high concentration IL-12 group was more than 12 months;in high concentration IL-2 + IL-12 group,1 mouse survived for 2 months and 4 mice survived for more than 12 months;in IL-12 + IL-15 group,only 1 mouse survived for more than 12 months.3.The number of NK cells in T-ALL mice before and after administration was detected:(1)the number of NK cells in leukemia mice was higher than that in normal mice,and the difference was statistically significant(P < 0.05)in peripheral blood,spleen and bone marrow;(2)the number of NK cells in T-ALL mice decreased with time,especially in bone marrow and spleen In the viscera,the differences between the groups were statistically significant(P < 0.05);(3)in the peripheral blood,the number of NK cells in T-ALL mice on the 10 th day of modeling(the 7th day of injection)was significantly reduced than that on the 3rd day before injection,and the difference was statistically significant(P < 0.05).Conclusion 1.The survival time of leukemia mice was significantly prolonged by appropriate cytokine injection,and the number of NK cells in leukemia mice was changed,especially in bone marrow and spleen.2.In this study,cytokines were injected into leukemia mice to provide new ideas and experimental basis for improving NK cell activity and NK cell immunotherapy.