Correlation And Comparison Between Vascular Endothelial Function And Special Performance Of Female Rowers At Different Levels

Research Objectives:By observing the changes of flow-mediated Dilation（FMD）,miR-21-5p,miR-155-5p levels and maximal oxygen uptake in brachial artery of rowers at different levels,the relationship between vascular endothelial function and special performance was discussed.It provides scientific basis for the application of relevant indexes in the assessment and monitoring of rower’s function.Research methods:（1）Research Object:In this study,20 female open rowing athletes and 6 ordinary students were selected as the research objects.According to their sports level,rowers were divided into 9 advanced group and 11 medium group.Ordinary students were selected as the control group（6,non-athletes）.The experiment was approved by the Ethics Committee（Approval No.2021024）.（2）Test indicators:general physical indicators:height,weight and BMI were detected by fitness tester;Vascular endothelial function:FMD,brachial artery quiet diameter and maximum dilated diameter were detected by vascular endothelial function detector.Hematological indicators:The expressions of Mir-21-5p and Mir-155-5p in circulating blood were detected by real-time fluorescence quantitative PCR.Sports ability and special achievements:maximal oxygen uptake,2km dynamometer,4km running,4km water rowing,vertical vertical jump,bench pull,bench press,weighted squat.Results:（1）General indicators:the height of rowing athletes at different levels was significantly higher than that of ordinary students（P<0.001）;The body weight of advanced group was significantly higher than that of ordinary students（P<0.01）.There was no significant difference in BMI among all groups（P>0.05）.（2）Vascular endothelial function:the vascular diastolic function（FMD）of brachial artery in advanced athletes was significantly higher than that in ordinary students（P<0.01）.The higher the exercise level was,the larger the brachial artery quiet diameter and maximum diameter were,but there was no significant difference between different athletes（P>0.05）,and there was no significant difference between athletes and ordinary students（P>0.05）.（3）Blood indicators:the expressions of miR-155-5p and miR-21-5p in circulating blood of high-level athletes were significantly lower than those of ordinary students（P<0.05 or P<0.01）;The expression of miR-21-5p in circulating blood of medium level athletes was lower than that of ordinary students,but there was no statistical significance（P>0.05）.（4）Athletic Ability and Special Achievements:（1）The special results reflecting the aerobic level of rowing athletes were maximal oxygen uptake,2km meter,4km running,4km water rowing.The maximal oxygen uptake of all rowing athletes was significantly higher than that of ordinary students（P<0.001）.The maximal oxygen uptake level of high level athletes was significantly higher than that of medium level athletes（P<0.001）.The results of 2km dynamometer and 4km water rowing were significantly different from those of medium level athletes（P<0.05 or P<0.01）.（2）The results of vertical vertical jump,bench pull,bench press and squat with load reflected the strength level.The results of bench pull,bench press and vertical vertical jump of advanced athletes were significantly different from those of medium athletes（P<0.05）.（5）Correlation analysis:（1）There was a significant positive correlation between miR-155-5p and miR-21-5p expression in circulating blood（r=0.7123,P<0.001）.（2）There was a high negative correlation between FMD and miR-155-5p expression（r=-0.7748,P<0.01）;The expression of miR-21-5p was negatively correlated with FMD（r=-0.5204,P<0.05）.（3）There was a highly negative correlation between FMD and 2km dynamometer performance（r=-0.7326,P<0.01）,and was negatively correlated with 4km running performance（r=-0.4992,P<0.05）,was negatively correlated with 4km water rowing performance（r=-0.6347,P<0.05）,was highly correlated with maximal oxygen uptake（r=0.9077,P<0.01）.（4）There was a positive correlation between FMD and vertical jump performance（r=0.7661,P<0.01）;There was a positive correlation between FMD and the results of pull-out（R=0.6879,P<0.01）;FMD was positively correlated with bench press performance（r=0.4490,P<0.05）;It was positively correlated with the performance of weight-bearing squat（R=0.4861,P<0.05）.（5）miR-155-5p was positively correlated with 4km running（r=0.5959,P<0.01）.It was positively correlated with 2km dynamometer performance（r=0.4514,P<0.05）;And the results of 4km water rowing were positively correlated（r=0.5513,P<0.05）.It was highly correlated with the expression of VO_2max（r=-0.7202,P<0.05）;It was highly negatively correlated with vertical jump performance（R=-0.7548,P<0.01）.Was negatively correlated with the grade of pull-down（R=-0.4986,P<0.05）（6）miR-21-5p was positively correlated with 4km running height（r=0.6519,P<0.01）;It was positively correlated with the height of 2km dynamometer（r=0.6141,P<0.01）.It was positively correlated with the height of 4km water rowing（r=0.7019,P<0.01）.It was correlated with the change of maximal oxygen uptake（r=-0.6156,P<0.05）;It was negatively correlated with vertical jump height（r=--0.5930,P<0.01）.Research Conclusions:（1）There was a high correlation between vascular endothelial diastolic function and special performance in female rowers of different levels,and FMD could be used as a non-invasive index to evaluate vascular endothelial diastolic function and aerobic endurance.（2）miR-21-5P and miR-155-5p level in circulating blood are highly negatively correlated with FMD,which can also be used as a potential biomarker for predicting athletic ability and specific performance.