| Objective: Myofascial pain syndrome（MPS）is a myogenic injury disease in which myofascial trigger point（MTr P）causes clinical symptoms such as pain and dysfunction.The cause of myofascial trigger point is due to the sudden appearance of continuous muscle fiber contraction after tissue injury or microtrauma,causing local energy loss and blocking local blood circulation,so that the ATP supply is insufficient.In this study,we observed the contents of MGF,Myo D and CD34 proteins,as well as the proliferation of muscle satellite cells and changes in microvessel density at the vastus medialis trigger point in MPS model rats.To investigate the mechanism of fasciotome in improving local muscle tissue injury and hypoxia-ischemia symptoms in MPS model rats.Methods: Fifty-six SD rats were randomly divided into 2 groups,16 rats in the blank group（NG）and 40 rats in the modeling group were randomly selected for model detection after modeling,and the remaining 32 rats were randomly divided into 16 rats in the model group（MG）and 16 rats in the treatment group（IG）.1 NG group was given grasping stimulation.（2）The MPS model was established by blunt impact combined with centrifugal running for eight weeks in the model group.The rats were hit with a heavy object with a kinetic energy of 2.352 J in the left vastus medialis muscle on Monday and downhill centrifugal running on an electric treadmill at-16 ° on Tuesday for 90 minutes,and then fed normally for four weeks and the model was detected.（3）MG group did not intervene in normal feeding,and fasciotome treatment was performed on the basis of successful IG modeling,three times a week,for a total of three weeks.In the first stage,8rats in NG,MG and IG groups were randomly sacrificed on the day before fasciotome treatment,and the contents of CD34,MGF and Myod in the left vastus medialis muscle were measured by ELISA.In the second stage,the contents of CD34,MGF and Myod in the left vastus medialis muscle of 8 rats in each group were randomly selected by ELISA after peripheral fasciotome treatment;the histomorphology was observed by HE stained sections,CD34 was detected by immunohistochemistry,and the microvessel density was measured by Weidner’s highest vascular density counting method.Results: Stage I: The results of ELISA before treatment: Compared with the blank group,the contents of CD34,MGF and Myod in the left vastus medialis muscle of rats in the model group and treatment group were significantly increased（P < 0.05）.Second stage:（1）ELISA results after treatment: The contents of CD34,MGF and Myod in the left vastus medialis muscle of rats in the model group and the treatment blank group were significantly higher than those in the model group（P < 0.05）.The contents of CD34,MGF and Myod in the left vastus medialis muscle of rats in the model treatment group were significantly higher than those in the model treatment group（P < 0.05）.（2）Immunohistochemical results: The mean optical density of the blank group and the model group was significantly higher（P < 0.05）.（3）Results of microvessel density count: The microvessel density was significantly lower in the model group than in the blank group（P< 0.05）,and significantly higher in the treatment group than in the blank group（P < 0.05）.（4）HE staining results: In the blank group,the morphology of myocytes was regular and closely arranged;the myofibers were orderly arranged without abnormal thickening or thinning.In the model group,the myocytes showed edema and inflammatory infiltration,the thickness of muscle fibers was different,and there were some fractures and twists and deformations.In the treatment group,most myocytes had been repaired,inflammatory infiltration was improved,muscle fibers still had some distortion,breakage was improved,and the arrangement was slightly regular.ELISA results of left vastus medialis muscle before and after treatment: The contents of CD34,MGF and Myod before treatment were significantly decreased（P < 0.05）.After treatment,the contents of CD34 and MGF did not increase significantly before treatment（P < 0.05）,and the content of Myod increased significantly（P < 0.05）.Conclusions: 1.Through experimental studies,it has been found that IASTM can increase microvessel density and improve the local hypoxia-ischemia environment of MPS by treating the trigger point of the left vastus medialis muscle around rats.2.IASTM promotes local muscle satellite cell proliferation at the trigger point to improve muscle repair ability,promote muscle tissue repair,and improve the state of damaged muscle injury through mechanical pressure. |
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