Effects Of Funneliformis Mosseae On Root Rot And Pathogenic Fungal Diversity In Continuous Cropping Soybeans

Continuous cropping for many years can lead to reductions in crop yields and agricultural product quality,which severely restricts the sustainable development of agriculture.The main factors that produce continuous cropping soybean obstacles include soil habitat deterioration,plant self toxicity,soil borne pathogens and so on,which will lead to crop yield reduction.Recently,scientists working in ecology and agricultural production have explored the effect of AM fungi in alleviating the challenges posed by continuous cropping.This study aims at investigation of interacting effects of Funneliformis mosseae(F.mosseae)on the root rot fungus community structure of soybean root systems and soil rhizophere at the incidencing of root rot stage under continuous cropping conditions.Overall,we aim to provide a theoretical basis for the future development of technology that utilizes AM fungi in the issues posed by continuous cropping.Nested-PCR-DGGE method was used to analyze soybean roots from 0-year,1-year and 3-year cropping soil samples after inoculation.The results showed that the diversity index and the abundance of root rot of different soybean roots were highest in the 3-year cropping soil samples and next came the 1-year and 0-year cropping soil samples.Fusarium oxysprum and Rhizoctonia solani were dominant in root systems in all two cultivars.The real-time fluorescence qunantitative PCR method was used to analyze investigation of interacting effects of Funneliformis mosseae(F.mosseae)on the root rot fungus Fusarium oxysprum DNA content and Rhizoctonia solani DNA content of soybean root systems.The shows that after F.mosseae inoculation,the Fusarium oxysprum DNA content and Rhizoctonia solani DNA content of different soybean roots were lowest than control group.In order to explain the relationship between F.mosseae continuous cropping soybean root rot fungi.Miseq high-throughput sequencing method was used to analyze soil rhizophere from 0-year,1-year and 3-year cropping soil samples after inoculation.The results showed that the diversity index and the abundance of root rot of different soybean rhizophere were highest in the 3-year cropping soil samples and next came the 1-year and 0-year cropping soil samples.Fusarium and Mortierella were dominant in soil rhizophere in all two cultivars.For the quantitative analysis of two root rot found,two soybean varieties were showed in 1 continuous cropping soil rhizosphere when Fusarium species quickly broke out,F.mosseae handle pouch mildew on its inhibition is not obvious,the Mortierella growth inhibition is more obvious,and in the soil rhizosphere of continuous cropping in 3 years F.mosseae,handle of the sac belonged to inhibit Fusarium,but on Mortierella inhibition is not obvious,so to clarify F.mosseae handle pouch mildew in different years of continuous cropping soil environment for the inhibitory effect of different root rot fungi,and on the other hand,F.mosseae stated in capsule by handle the environment to form a synergistic effect to inhibit the other root rot pathogen fungi and some pathogenic fungi.In conclusion,F.mosseae on the root rot fungus community structure of soybean root systems and soil rhizophere at the incidencing of root rot stage under continuous cropping conditions..At the same time,it is proved that F.mosseae handle pouch mildew on the superiority of root and soil rhizosphere pathogenic fungi with ecological antagonistic relationship.