Effects And Mechanism Of High-frequency Electrical Stimulation On Epileptiform Discharges In The CA3 Region Of Rat Hippocampal Slices

Epilepsy is a chronic neurological disease with high incidence,which arises from abnormal synchronous neuronal firing of brain groups,and at the onset of the disease,the human body will experience symptoms such as loss of consciousness,generalized tonic or convulsions.Among them,temporal lobe epilepsy,as a representative of epilepsy disease,its abnormal discharge starts in the hippocampus region,and the surgical risk is large and easy to produce tolerance to anti epileptic drugs,has been receiving the attention of numerous research scholars.High frequency electrical stimulation has been shown to be a powerful means of suppressing temporal lobe seizures in several clinical and experimental studies,but its mechanism of action remains to be explored.In addition,the effects of high-frequency electrical stimulation with different stimulus pulse numbers on various parameters,such as the place of seizure discharge initiation,the rate of transmission,the direction of transmission,and the power spectral density in the hippocampus,also need to be clarified.Therefore,different modes of high-frequency electrical stimulation were used in this study to explore the effects and action mechanisms on epileptiform discharges in view of the above issues.First,the artificial cerebrospinal fluid(CSF)with low Mg2 +(i.e,ACSF)was successfully induced to model epileptiform discharges on hippocampal isolated slices,electrophysiological signals were recorded by electrode array system,and the experimental results showed that epileptiform discharges started in CA3 region,and their complete discharge process included inter ictal discharges(IID)and ictal discharges Discharges,ID);secondly,high-frequency electrical stimulation was applied in the hippocampal CA3 region to contrast the changes of epileptiform discharges before and after electrical stimulation.The results indicated that high-frequency electrical stimulation could obviously inhibit epileptiform discharges and did not disrupt the neural pathways of brain tissue.In addition,we further examined the effects of different durations,intensities,different stimulus pulse numbers,and different frequencies of high-frequency electrical stimulation on epileptiform discharges,and the results showed that high-frequency electrical stimulation(130 Hz,square wave,900 pulses,150The volt time increased to 171.93%,the interval time decreased to 56.48%,and the magnitude of ID decreased to 54.77%.Finally,the present study was designed to combine pharmacological experiments to clarify the mechanism underlying the response of high-frequency electrical stimulation to epileptiform epileptiform activity by using the gamma aminobutyric acid(GABA)receptor inhibitor bicuculline(bicuculline,BIC)demonstrated that high-frequency electrical stimulation was effective in suppressing epilepsy by activating GABA receptors,which in turn affected chloride channel current firing,producing some kind of inhibitory change within the cell.In this study,we firstly demonstrate that high-frequency electrical stimulation can effectively inhibit epileptic discharges,and the protocol of high-frequency electrical stimulation is optimized,and further explore the mechanism of high-frequency electrical stimulation,the research results of this paper provide some data accumulation and theoretical support for the clinical application of high-frequency electrical stimulation for the treatment of epilepsy.