Study On Chemical Constituents,Antibacterial Activity And Application Of German Chamomile

German chamomile is native to the northern and western parts of Eurasia,and is also grown in Xinjiang.Based on the literature research on the pharmacological activity of Asteraceae,this experiment was conducted to analyse and identify the chemical composition of its volatile oil and pure dew,evaluate its quality,investigate and determine the effective parts of its alcoholic extracts for antibacterial and active substance basis,and incorporate the effective parts of antibacterial into the soap with the research results of the group to clarify the potential application of German Chamomile as a natural antibacterial and antiseptic agent.The potential applications of German Chamomile as a natural antibacterial and antiseptic agent were identified.The main research results were as follows:(1)The volatile oil and pure dew from the above-ground parts of German chamomile were extracted by hydrodistillation.The results showed that the volatile compounds of German chamomile were more abundant and accounted for a larger proportion.The results showed that the volatile oils and pure dew of German chamomile were all dominated by olefins.(2)The inhibitory effects of different parts of the ethanol extracts from German chamomile on Staphylococcus aureus,Escherichia coli,Bacillus subtilis and Pseudomonas aeruginosa were investigated.The minimum inhibitory concentration and the minimum bactericidal concentration were determined by measuring the size of inhibitory zone and two-fold dilution method,and the effective inhibitory part was determined.The results showed that the four solvent extracts of German chamomile after alcohol extraction had stronger bacteriostatic effect on gram-positive bacteria than gram-negative bacteria,and the ethyl acetate extract had the best bacteriostatic activity on the four bacteria,which was the effective bacteriostatic part of German chamomile.This part achieved the bacteriostatic effect by destroying the cell morphology,and affecting cell wall integrity and cell membrane permeability.(3)The chemical constituents of ethyl acetate extract were identified by liquid-mass spectrometry.The results showed that 24 compounds could be detected,and 4 compounds were identified,which were cinnamic acid,3,5-dimethoxyphenylacetic acid,7-methoxycoumarin and apigenin.The antibacterial soaps were made from the effective antibacterial part extracted from German chamomile alcohol.The comprehensive evaluation score of the soaps was high,the physical and chemical indexes could meet the requirements of the corresponding national standards and has a good antibacterial effect.