| Among the numerous methods in biological and medical research,optical probes have the advantages of low biotoxicity,sensitivity,and fast response speed,enabling the real-time detection and dynamic imaging of target analytes in cells and in vivo.Optical probes are essential means for studying physiological processes and monitoring pathological changes.As one kind of optical probes,lanthanide complex-based bioprobes exhibit various advantages,including sharp and narrow emission bands,long luminescence lifetime,large antenna-generated Stokes shifts,and excellent photostability,which enables them to meet the requirements of practical utilities in living systems.Therefore,developing stable and versatile lanthanide complex-based bioprobes are promising candidates for biodetection,bioimaging,and disease theranostics.Functionalization of lanthanide complex-based bioprobes is generally achieved through ligand modification and complex molecule design.In this thesis,we introduce the technology of supramolecular chemistry to develop new strategies of functionalizing lanthanide complexes for construction of novel bioprobes.We constructed a nano micellar probe assembled from lanthanide complexes driven by hydrophobicity and a luminescent probe based on cyclodextrin-lanthanide complex with the ability of host-guest recognition,respectively.The experimental results have shown that these two functionalized bioprobes based on lanthanide complex synthesized via supramolecular approaches,both have good cell imaging capabilities.The specific content of this thesis is divided as follows:Chapter One:We briefly introduce the related concepts of rare earth elements and lanthanide complexes,summarizing the applications of luminescent lanthanide complexes as biological probes for fluorescence sensing and imaging.Recent research advances are mainly discussed by the following three aspects:molecular probes based on lanthanide complex,probes based on lanthanide coordination polymer,and probes based on self-assemblies of lanthanide complex.Chapter Two:The assembled process of amphiphilic lanthanide complexes,which enables the formation of nano micelles homogeneously dispersed in the aqueous phase and reduces fluorescence quenching,is a new method for synthesizing lanthanide complex bioprobes.In this part of the work,we have successfully synthesized the self-assembly of amphiphilic lanthanide complex Eu(THA12)2THBphen and its co-assembly with HPS molecule by hydrothermal method.Both nano luminescent probes assembled in this work exhibit good temperature-responsive properties and the ability for intracellular mitochondrial-targeted imaging.The co-assembly has the capability of ratiometric detection and multichannel imaging.Chapter Three:Specific detection of proteins with unknown structures by fluorescent probes generally through covalent attachment of fluorophores to antibodies,which are immunologically recognized with antigenic proteins,while the process of chemical modification can easily cause antibodies to lose activity.Therefore,we propose a new method to construct composite probes by combining lanthanide complex bioprobes with antibodies via host-guest recognition.Cyclodextrin derivatives are used as ligands to synthesize cyclodextrin-lanthanide complexes,i.e.,Eu（β-CD-HOP）TTA3py.This complex has good ability for cell imaging,and the cavity ofβ-cyclodextrin in the complex can participate in host-guest recognition as a host.The cavities of cyclodextrin can assemble with amino acid residues on the surface of antibodies via host-guest recognition to construct luminescent probes as the formation of lanthanide complexes-cyclodextrin-antibody composite for imaging of intracellular proteins.Chapter Four:The summary and perspectives of this thesis. |
