| Optical microscope has played an indelible role in promoting the development of science and technology.The continuous development of science and technology also puts forward higher and higher requirements for the resolution of optical microscope.However,the traditional optical microscope is limited by the Abbe diffraction limit.The highest resolution that can be achieved under white light is only about 200～300 nm,which restricts the further exploration of the micro world.Among all kinds of super-resolution imaging technologies that break through the diffraction limit,the super-resolution imaging technology based on micro spherical superlens has become one of the current research hotspots because of its advantages such as lossless,real-time and environment compatible with traditional optical imaging.However,due to the small imaging field of vision,the development of imaging system using super-resolution imaging technology based on microsphere superlens is challenged.In this thesis,based on the existing microsphere super resolution microscope in the laboratory,a control system for microsphere super resolution microscope is proposed and designed.Through scanning and image acquisition,image processing and splicing,a large range scanning imaging of the designated area is achieved.The main research contents of this thesis are as follows:(1)The overall design of the system: For the micro sphere super resolution microscope,the functions that the system needs to achieve are divided into two overall functional modules: the motion platform control and image acquisition module and the super resolution image processing module.The overall functional module is divided into seven sub modules,and the composition of the control system is described.(2)The design of the motion platform control and image acquisition module: First,the research content of this part selects the scanning image acquisition path and the running time sequence of the camera and the mobile platform.Then,the method of establishing the relationship between the mobile platform displacement and the captured image pixels through image acquisition and registration,and then accurately calculating the scanning step,and the method of calculating and compensating the mobile platform position deviation angle are studied.Finally,based on the self sensing probe assembly in the micro sphere super resolution microscope,a position feedback control method of micro sphere super lens based on PID is designed.(3)The design of discrete super-resolution image processing module: For the discrete images scanned and collected in the above process,the noise suppression method of discrete images is studied in combination with its characteristics,and for the problem of uneven image brightness in the scanned and collected discrete images,a method of discrete image brightness homogenization is proposed;Finally,the discrete image fusion algorithm is studied.(4)Software design of control system of microsphere super resolution microscope:Based on LabVIEW software,the scanning imaging,real-time imaging and white light fluorescence co imaging image generation are realized by using the algorithm designed above.(5)Research on imaging characteristics of micro spherical superlens in continuous variable index background medium under inclined illumination: The upper computer software developed in this research is used to control the micro spherical super-resolution microscope to conduct experimental and simulation research on imaging characteristics of micro spherical superlens in continuous variable index background medium under inclined illumination.Through experiments on the imaging effects of microspheres in different refractive index background media,and FDTD simulation of microspheres with the same experimental parameters,the relationship between the refractive index of background media and the imaging characteristics of microspheres under inclined illumination is obtained,which broadens the application range of microsphere superlens imaging. |
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