| Salt and drought stress are important factors affecting the growth of wheat,and the phospholipid signaling plays a vital role in regulating plant responses to abiotic stresses.Phosphatidylinositol-dependent phospholipase C (PI-PLC,PLC) is a key signaling molecules of phospholipid signaling,which is involved in plant growth and development and stress response in many plants.Previously,both the qRT-PCR and Western bolt results showed that the expression of TaPLC1 and TaPLC2 were induced by salt or drought stress.Moreover,TaPLC participated in wheat seedling growth and responds to salt or drought stress with PLC inhibitors treatment.With the wheat genome sequence unveiled,we performed genome-wide analysis to identify the wheat phosphatidylinositol-specific phospholipase C.Firstly,we used bioinformatics to identify the TaPLC family at the whole genome level,and analyzed the structure and evolution characteristics of the TaPLCs gene in wheat.Secondly,we explored their relative transcript level in various tissue and the expression pattern under drought and salt stress.Finally, we confirmed the function of TaPLCs on responsing in drought stress in Arabidopsis.The results as follows:1.Based on the genome database,TaPLCs genes were identified from Triticum aestivum,using rice and Arabidopsis PLC genes as reference sequences.A total of 11 TaPLC members within the heterologous hexaploid wheat genome were identified and analysis.All TaPLCs have conserved X and Y catalytic domains and C2 domains,just like other plant PLCs.According to chromosome distribution and phylogenetic analysis,11 TaPLCs were divided into 4 groups,namely TaPLC1A,TaPLC1D,TaPLC2A,TaPLC2B,TaPLC2D,TaPLC3A,TaPLC3B,TaPLC3D and TaPLC4A,TaPLC4B,TaPLC4D.They are all orthologous genes of plant PLCs in plants,but there are some differences in evolution.2.According to the data of RNA-seq and qRT-PCR,our findings reveal that each TaPLCs has a unique tissue expression pattern,and they are expressed mainly in root,stems,leaves,ears and grains.Subcellular localization results showed that these proteins were located in the mitochondria,chloroplasts and cytoplasm,while TaPLC1D,TaPLC2B and TaPLC4A were also detected in cell membrane.3.Further analysis of cis-regulatory elements elucidated that these members of TaPLCs family shared similar elements,such as the phytohormone and the stress response cis-elements.The expression of TaPLC1D, TaPLC2B, TaPLC3A and TaPLC4A was significantly induced by salt or drought stress using qRT-PCR.Under drought stress,the expression of TaPLCs showed a trend of first increasing and then decreasing with the stress time,reaching a peak at 6～12 h,among which TaPLC1D and TaPLC2B showed the most significant changes,increasing by 30 and 20 times respectively.Similarly,the salt stress could also active the expression of TaPL2B and TaPLC3A dramatically,which increased by 28 and 23 times respectively.4.Interestingly,we found that TaPLC1D and TaPLC2B were involved to drought stress in different mechanisms.We obtained the OETaPLC1D and OETaPLC2B transgenic lines through genetic transformation of Arabidopsis thaliana.The OETaPLC2B plants could enhance the drought tolerance,while OETaPLC1D plants were involved in drought escape. |
