Application Of Counting Algorithm Based On Interference Elimination In Pheromone Trapping Tea Green Leafhopper

Pheromones are a class of compounds that can be recognized by individual insects and used to search for food or heterosexual mating in the natural environment.In recent years,the application of pheromone in pest control has been rapidly developed,and Empoasca flavescens F.as a harmful and difficult pest in tea garden,its green control problem remains to be solved.A combination of Gas adsorption platform and GC-MS was developed to separate and identify the volatile compounds in order to effectively prevent and control leafhopper.This paper presents a program algorithm for counting leafhoppers trapped on sticky board based on interference elimination,which is used to count and evaluate the control effect.The main contents and conclusions are as follows:(1)A kind of adsorption platform for collecting volatile gas was set up.The super Q and Porapak Q adsorption columns were loaded to adsorb the volatile compounds of three plants(Flemingia,French Marigold,Marigold)and the components were analyzed by GC-MS.In this study,using Flemingia as experimental material,53 components were adsorbed by Porapak Q and 45 components were adsorbed by super Q.Using French Marigold as experimental material,Porapak Q adsorbed 36 volatile components,super Q adsorbed 26 components,a total of 18 same components.Using Marigold as material,Porapak Q adsorbed 36 components,super Q also adsorbed 34 components,18 same components.(2)This thesis presents an automatic counting program algorithm for trapping and killing leafhoppers on the trap board.After background segmentation,the number of leafhoppers can be obtained by removing the corresponding interference area,and the relative error is about 5%.It has a certain practical value to use in the process of trapping and killing leafhopper on the measuring trap board in the field experiment.It provides a convenient method for the identification and counting of leafhoppers.(3)The results showed that French Marigold and Marigold significantly repelled leafhoppers,and Flemingia significantly attracted leafhoppers.Then,21 repellent components and 40 attractant components were selected from the volatile compounds of repellent plants French Marigold and Marigold,attractant plants Flemingia,tea leaves and tea flowers.A series of repellents and attractants were prepared to trap leafhopper in tea garden.(4)Three kinds of control devices were set up and the effects were compared:the attractant core was attached to the trap board to trap and kill leafhoppers;the repellent core was bound to the tea clump to repel leafhoppers,and the trap board was hung over the tea bush to trap and kill leafhoppers;the repellent core was fixed on the tea clump to repel leafhoppers,and the trap board carrying the attractant core was hung over the tea bush to form a "push-pull" trap.Through a series of field experiments,it was found that the "push-pull" trap was more effective than the single attractant or repellent trap.The trapping and killing area composed of 5 kinds of attractants,2 kinds of repellents and "pushpull" traps showed a corrected leafhopper population decline rate of 56%.The gas adsorption platform has the functions of pressure stabilization and filtration,which solves the problems of unstable air flow and impurity pollution,and is easy to be disassembled and installed.The results show that compared with other researches on volatile matter of Flemingia,more components can be absorbed.The volatiles of repellent and attractant plants contain repellent and attractant components respectively.Repellents or attractants with stronger bioactive components screened by indoor and outdoor bioassay can show stronger trapping effect in the field.The "push-pull" trapping device composed of suitable repellent and attractant can obtain excellent trapping effect,which is worthy of further demonstration and application.During the field experiment,the leafhopper counting algorithm has the advantages of fast recognition speed and high accuracy,which can effectively replace the manual counting and provide a convenient method for sticky board pest counting.