Sequencing And Analysis Of Full-length Transcriptome In Sturgeons

Sturgeons are polyploid fish,there are four kinds of ploidy,tetraploid,octoploid,dodecaploid and sixteen-ploid.Tetraploid sturgeons have ~120 chromosomes,octoploid sturgeons have ~240 chromosomes,dedcaploid sturgeons have ~360 chromosomes,sixteen-ploid sturgeons have ~480 chromosomes.The situation of sturgeon chromosomes is complicated,not only sturgeons have a large number of chromosomes,but also their chromosomes are composed of macrochromosomes and microchromosomes.So it is hard to get sturgeons high quality genomes.Compared with the second-generation sequencing technology,the length of reads that the third-generation sequencing technology sequence is longer,and we can analyse the full-length transcriptome without assembly.Because of this advantage we choose Pac Bio sequencing platform to sequence full-length transcriptoms of three main cultured sturgeons in China,which are Huso dauricus,Acipenser schrenckii and Acipenser stellatus.We use high quality full-length transcriptomes to annotate the function of genes,analyse simple sequence repeat(SSR)and single nucleotide polymorphism(SNP),predict coding sequence(CDS)and transcription factors(TFs),we also constructed a fossil-calibrated phylogenetic tree,and we can know about the divergence time of three stugeons.This study provides a reference for future study of divergence time and gene annotation.