Effects Of Sodium Selenite On Semen Quality,Sperm Mitochondrial Activity And Liquid Storage In Sheep

The objective of this study was to investigate the effects of dietary selenium on semen quality,antioxidant activity,sperm mitochondrial activity,energy metabolism and GLUTs protein expression in sheep.After liquid storage at 5℃ for 24 h,48 h and 72 h,these indexes were also evaluated.The experiment consists of two parts:(1)Twenty-four healthy doper rams were selected and divided into two treatment groups: the control group and Se treatment group.They were fed with basal diet and the basal diet supplemented with 0.5 mg/kg sodium selenite,respectively.At the end of the feeding period(90 days),sperm motility,motive parameters,mitochondrial activity,antioxidant enzyme activity and the abundance of GLUTs protein were evaluated;(2)After semen of these two groups were stored at 5℃ for 0 h,24 h,48 h and 72 h,the above indexes were also detected.The results showed that sperm motility and motility increased by 5.45% and 4.26%respectively,and sperm mitochondrial activity also significantly(P<0.05)increased.In addition,SOD activity increased by 13.39%,MDA content decreased by 14.59%,T-AOC increased by21.59%.The abundance of GLUT3 and GLUT8 in sperm of experimental group were significantly(P<0.05)increased.Although the sperm motility and motivate parameters were significantly(P<0.05)decreased after 72 h of liquid storage at 5℃,the indexes in the Se treatment group was still higher than those in the control group.The activity of T-AOC and SOD was significantly(P<0.05)higher than that in the control group,but MDA content was lower than that of the control group.The result indicates that dietary Se could enhance the antioxidant status and energy metabolism of sperm and improve the sperm motility.With the elongation of liquid storage period,sperm motility,mitochondrial activity,antioxidant enzyme activity and GLUT protein expression in the Se treatment group were higher than those in the control group.Se can protect the integrity of sperm membrane and prolongs the survival time of sperm.Semen quality of liquid storage at 5℃ was affected by Se supplementation in diet.