Analysis Of Microbial Community Characteristics In Rhizosphere Soil Of Main Lycium Barbarum In Ningxia

In order to research the microbial community characteristics in rhizosphere soil of main Lycium barbarum in Ningxia,Ningqi 1(NQ1),Ningqi 5(NQ5),Ningqi 7(NQ7),Ningqi 9(NQ9),Black Chinese wolfberry(BCW)and Yellow Chinese wolfberry(YCW)were used as research materials.MiseqPE300 high-throughput sequencing method was used to analyze the microbial community diversity of bacteria(16S),archaea and fungi(ITS1)in rhizosphere soil.QPCR was used to excavate phoD and nifH functional gene diversity in rhizosphere soil,and analyze the diversity of species composition,richness and community structure.Correlation analysis was made between microbial community and soil factors.The results are as follows:(1)The main bacterial categories were Proteobacteria(23.06%～34.17%),Actinobacteria(15.21%～25.18%),Chlorobacteria(9.12%～17.91%),Gemmatimonadota(8.088%～13.91%)and Acidobacteriota(6.82%～12.00%).The population abundance is NQ9>NQ5>NQ1>BCW>YCW>NQ7,and the diversity is NQ9>NQ7>NQ1>NQ5>BCW>YCW.At the genus level of bacterial community,unidentified and unidentified bacteria were the main genera,indicating that there were most new species to be studied at the genus level.MND1,TX1A-55 and Woeseia were the dominant genera.FastUniFrac analysis showed that NQ7 and YCW had the largest difference in community structure,while NQ9 and BCW had the smallest difference.(2)The main categories of archaea were Crenarchaeota(30.59%-69.77%).Thermoplasmatota(28.73%-59.16%)and Halobacterota(0.63%-9.84%).The population abundance is YCW>NQ9>BCW>NQ1>NQ5>NQ7.The diversity is YCW>NQ9>BCW>NQ1>NQ5>NQ7.At the genus level of the archaea community,unidentified and unidentified bacteria are the main genera,indicating that there are most new species to be studied at the genus level of the archaea community.Candidatus_Nitrocosmicus and Candidatus_Nitrososphaera were the dominant genera.FastUniFrac analysis showed that NQ1 and NQ5 had the largest difference in community structure,while NQ9 and YCW had the smallest difference.(3)The main categories of fungi are Ascomycota(50.10%-83.15%),Unidentified(6.86%-12.20%),Mortierellomycota(1.91%-16.16%)and Basidiomycota(1.54%-34.03%).The population richness is NQ1>NQ9>NQ7>NQ5>BCW>YCW,and the diversity is NQ9>NQ7>NQ5>NQ1>YCW>BCW.In the genus level of fungal community,unidentified,Plectosphaerella,Lophotrichus,Acremonium and Mortierella were the main genera,in which the proportion of Unidentified was 36.48%～59.91%,indicating that there were most new species to be studied on the genus level of fungal community.Plectosphaerella、Lophotrichus and Acremonium are the dominant bacteria.FastUniFrac analysis showed that NQ5 and YCW had the largest difference in community structure,while NQ1 and NQ9 had the smallest difference.(4)nifH gene-related nitrogen-fixing bacteria were Proteobacteria,Verrucomicrobia,Firmicutes,unidentified and Actinobacteria.The population diversity is NQ9>NQ1>YCW>BCW>NQ5>NQ7,and the richness is NQ1>NQ9>BCW>NQ5>YCW>NQ7.In terms of genus level of nifH gene related soil nitrogen-fixing bacteria community,Desulfuromonas,Unidentified and Skermanella were the dominant species.PCoA analysis showed that NQ1 and NQ9,BCW and YCW had similar microbial communities.NQ5 and YCW microbial communities were different.The difference between NQ7 and other cultivars was great.(5)PhoD gene-related phote-soluble bacteria mainly included Actinobacteria,Unidentified and Proteobacteria.The population diversity is:NQ9>YCW>NQ7>NQ5>NQ1>BCW,and the diversity is YCW>NQ9>NQ5>NQ7>NQ1>BCW.In the genus level of phoD gene related phosphorus-solubilizing bacteria community,Jiangella was the dominant bacterium.Unidentified and Other accounted for 47.28%-90.05%,indicating that phoD gene-associated phodp-soluting bacteria still have most new species composition to be studied at the genus level.PCoA analysis showed that the microbial communities of NQ7 and other cultivars were significantly different.NQ1,NQ5,NQ9,BCW and YCW had little difference in microbial communities.(6)Correlation analysis showed that soil environmental factors were the key factors affecting rhizosphere soil microbial community structure of six different lycium barbarum cultivars.The diversity of rhizosphere soil bacteria,archaea,fungi,nifH gene-related nitrogen-fixing bacteria and phoD gene-related phosphorus-soluting bacteria was significantly different between species and within species.