| Soybean(Glycine max)is an important crop in China for food,oil,and animal feed,providing rich sources of plant protein and energy for humans and animals.Currently,China’s demand for soybean is increasing year by year,while domestic soybean production has been relatively low,resulting in a large demand for soybean imports,which is a constraint on the international soybean market and a threat to local food security.Therefore,improving the yield of soybean and reducing dependence on imports is particularly important.Leguminous plants are extremely sensitive to drought,and soybeans are among the most sensitive.The root system adapt to drought stress by changing root structure and distribution.Auxin plays an important role in the development of plant roots,especially in lateral root development.The VAS1 gene encodes a pyridoxal phosphate(PLP)-dependent aminotransferase that converts indole3-pyruvate(IPA)to tryptophan(Trp)to regulate the concentration of indoleacetic acid(IAA)and thus regulate plant growth and development.This study identified and analyzed soybean Gm VAS1 gene family members using bioinformatics methods,and analyzed the expression patterns of the GmVAS1 gene family.After cloning the Gm VAS1-1 gene,a series of transgenic Arabidopsis were constructed.The phenotype analysis revealed that GmVAS1-1 participates in lateral root development.The main results of the study are as follows:1.Bioinformatics analysis of GmVAS1 gene family in soybeanTwo gene members of the GmVAS1 family were identified from the soybean genome using bioinformatics methods,and both were found to be relatively conserved in evolution.They are closely related to leguminous plants.Gm VAS1 family genes were expressed in different developmental stages of soybean seeds,but the highest expression level was observed in the endosperm.Abiotic stress-induced elements,hormone-responsive elements,and light-responsive elements enriched in the promoter region.qRT-PCR results showed that this gene family responded to drought and salt stresses.2.Identification of interacting factors and expression regulatory factors of GmVAS1－1Using yeast two-hybrid and yeast one-hybrid methods,the interacting factors and expression regulatory factors of GmVAS1-1 were identified.The results showed that the interacting proteins of GmVAS1-1 are mainly related to glycolysis,gluconeogenesis,and the pentose phosphate pathways;the transcriptional regulatory factors mainly include SWI/SNF complex and BPC1 transcription factor,etc.3.GmVAS1-1 is involved in regulating lateral root developmentBy constructing transgenic Arabidopsis materials and observing root phenotypes of wild-type Arabidopsis Col-0,mutant vas1,overexpression line Gm VAS1-Col-0,and complementation line GmVAS1-vas1,it was found that the number of lateral roots in vas1 mutant was significantly higher than that in the wild type,while the number of lateral roots in the complementation line GmVAS1-vas1 was significantly lower than the vas1 mutant.The number of lateral roots in Gm VAS1-Col-0 overexpression line was significantly lower than that of the wild type.Through analysis of vas1 and Col-0 transcriptome data,a total of 904 differentially expressed genes were obtained,of which 430 genes were upregulated and 474 genes were downregulated.Among them,several genes associated with plant hormones and lateral root development genes were identified,such as auxin response factors ARF18 and ARF14,abscisic acid receptor PYL1,auxin transport protein BIG,etc. |
