Effects Of Compound Chinese Herbal Preparation On Production Performance And Intestinal Barrier Of Meat Rabbits

The purpose of this experiment was to study the effects of compound Chinese herbal medicine preparation "Shanhuasu" added to the diet on the production performance,intestinal barrier,cecal flora and economic benefits of meat rabbits.288 healthy weaned meat rabbits(Laiwu black rabbits)with similar weight were randomly divided into 4 treatments,4 replicates for each treatment and 24 rabbits for each repetition.The control group was the basic diet,and the other three treatments added 750 mg/kg Chinese herbal medicine preparation and 1,500mg/kg Chinese herbal medicine preparation to the basic diet.The test results are as follows:(1)After adding compound Chinese herbal medicine preparation "Shanhuasu" to the diet,the mortality rate of meat rabbits decreased;The average daily feed intake of meat rabbits was significantly increased(P < 0.05)when the compound Chinese herbal medicine preparation "Shanhuasu" was added to the diet.The feed supplemented with compound Chinese herbal medicine preparation "Shanhuasu" significantly increased the live weight,full bore rate and half bore rate of meat rabbits before slaughter(P < 0.01);After adding compound Chinese herbal medicine preparation "Shanhuasu" to the diet,the liver weight of meat rabbits was significantly increased(P < 0.05).After adding compound Chinese herbal medicine preparation "Shanhuasu" to the diet,the muscle redness(a*)was significantly reduced(P < 0.05).(2)After adding compound Chinese herbal medicine preparation "Shanhuasu" to the diet,the 750 mg/kg compound Chinese herbal medicine preparation group significantly increased the gene expression level of ZO-2 in ileum(P < 0.05).The 750 mg/kg compound Chinese herbal medicine preparation group significantly increased the gene expression level of IL-2 in ileum(P < 0.05),and significantly increased the gene expression level of SIg A in duodenum(P <0.01);The addition of compound Chinese herbal medicine to the diet significantly increased the expression level of SIg A gene in the ileum(P < 0.05).The 750 mg/kg compound Chinese herbal medicine preparation group significantly increased the gene expression level of Bcl-2 in ileum(P < 0.05).The 750 mg/kg compound Chinese herbal medicine preparation group significantly increased the expression level of GPx1 in the ileum(P < 0.01).(3)After adding compound Chinese herbal medicine preparation "Shanhuasu" to the diet,there was a significant increase in the sobs and ace indices of the intestinal microbiota of meat rabbits(P < 0.05),indicating an increase in the abundance of intestinal microbiota.The dominant phyla were Firmicutes and Bacteroides.The 1,500 mg/kg compound Chinese herbal medicine preparation group showed significantly differences in microbiota compared to the other two groups.(4)After adding compound Chinese herbal medicine preparation "Shanhuasu" to the diet,the profit of each group was higher than that of the control group,and the profit of adding 750mg/kg compound Chinese herbal medicine preparation was the highest compared with other groups.Based on the above results,the meat quality of meat rabbits was improved by adding compound Chinese herbal medicine preparation "Shanhuasu" to the diet.The compound Chinese herbal medicine preparation "Shanhuasu" mainly acts on the ileum and duodenum of meat rabbits,can improve the intestinal barrier function,inhibit intestinal cell apoptosis,improve the intestinal antioxidant capacity and improve the intestinal flora structure.And then reduce the mortality of meat rabbits.Combined with the economic benefit analysis,the recommended addition amount of compound Chinese herbal medicine preparation "Shanhuasu" is 750 mg/kg.