| The skin insulates the body’s internal environment from its external environment,protecting the homeostasis of the internal environment,preventing water loss,and creating an important barrier.When skin integrity is compromised,the body activates various protective mechanisms,including blood clotting,to allow the wound to heal.First,a skin irritation test is conducted to test whether the drug is irritating.Next,non-irritant drugs were selected for drug screening test,and the drug with the highest wound healing rate was selected for wound healing test in canines.Test contents:(1)Drug screening test:schisandra ethyl gel,shikonin gel and Marber gel were prepared by Carbomer gel,and then skin irritation test and rabbit skin healing test were conducted.1)Skin irritation test:Four test areas were established on both sides of the middle back line of rabbits,each of which was a rectangle of 6 cm~2(3 cm long and 2 cm wide).After simulating skin injury,carbomer gel,schisandryl gel,shiverin gel and Marb gel were applied to the test area respectively.The changes of injury sites were observed and recorded at 0 h,12 h,24 h and 48 h at the beginning of the experiment.2)Rabbit healing rate test:Six round acute defect skin wounds were established on both sides of the dorsal midline of rabbits,3 on each side with a diameter of 10mm,from head to tail,from left to right.They were successively divided into control group,solvent group(0.5m L carbomer gel),positive group(0.5m L Bailingjin prescription),Mabob group(0.5m L Mabob gel),schisandrin ethyl group(0.5m L schisandrin ethyl gel)and shikonin group(0.5m L shikonin gel).The wound healing rate was calculated to determine the drug with the highest healing rate.(2)Dog wound healing test:Sixteen dogs were selected and 4 wound models were established on the back of each dog,from head to tail,from left to right,and they were divided into control group,solvent group,horse group and positive group,with the same dose as the rabbit skin healing test.The overall state,wound healing,wound healing rate,wound tissue structure and change,blood flow rate,oxidative stress index,growth factor level and inflammatory cytokines were observed immediately after the operation,on day 7,14,21 and 28.Results:(1)Skin irritation test results:schisandra ethyl gel,schisandrin gel,Mabo gel and Carbomer gel had no skin irritation.(2)Results of rabbit skin healing test:on the 3rd,6th,9th and12th day after surgery,the wound healing rate of Mappa group and positive group was higher than that of control group,solvent group,schisandrin ethyl group and schisandrin group,and the difference was significant(p<0.05);There was no significant difference between the solvent group and the control group(p>0.05).(3)Results of canine wound healing test:on the 7th,14th,21st and 28th days after surgery,the wound healing rate of Mabo group and positive group was higher than that of control group,and the difference was significant(p<0.05);There was no significant difference between the solvent group and the control group(p>0.05);HE staining and Masson staining showed that the Mabob group and positive group were better than the control group and solvent group at each time point.There was almost no difference in the average perfusion volume of all groups immediately after surgery.On day 7,14,21 and 28 after surgery,the average perfusion volume of Mabo group and positive group was higher than that of control group and solvent group,and the difference was significant(p<0.05);The results of oxidative stress detection,growth factor expression and inflammatory factor detection all proved that the effect of promoting healing in the Ma Bo group and positive group was significantly better than that in the control group and solvent group,and the difference was significant(p<0.05).To sum up:(1)The results of drug screening showed that schisandrin ethyl gel,schisandrin gel and Marb gel had no irritation on rabbit skin;The promoting effect of the horse herb on rabbit skin healing was better than that of schisandrin and shikonin.(2)The skin healing test of dogs showed that horsehair can accelerate the reepithelialization of the wound,increase the content of collagen fibers,increase the average perfusion volume of blood flow in the wound tissue,reduce the oxidative stress of the wound skin tissue,and shorten the healing time of the wound. |
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