| Objective:To establish the mode of rats with chronic heart failure and observe the impacts of shenlijia granular on the cardiac function,urine volume,cardiac muscle tissue pathomorphology and electrolyte of rats with chronic heart failure induced by ADRiamycin;to test,with the ELISA method,the expression levels and alteration trend of AVP and AQP-2 of rats with heart failure after their administration of shenlijia granular and to explore the anti-heart-failure function of shenlijia granular from the perspective of aqueous liquid retention.Methods:Chose 135 male Wistar rats with their weights from 180 to 200 g and preliminarily fed them for one week,then randomly chose 15 rats constituting blank group after weighing each of them,and injected the rest 1 20 rats with 2 mg/ml ADRiamycin solution,so as to establish the mode of the animal with chronic heart failure.Each one of the 120 rats received intraperitoneal injection,with the dosage,frequency and duration of 1.5 mg/kg,twice a week and seven weeks in total,and each rat of the blank group was inj ected with the same volume of normal saline,with the duration of seven weeks in total.The rats of which the mode had been successfully established were divided into six groups two weeks after their last administration,so there were seven groups in total,including blank group,mode group,Traditional Chinese Medicine control group(Qiliqiangxin),Western medicine control group(furosemide),shenlijia granular low-dosage group,shenlijia granular middle-dosage group and shenlijia granular high-dosage group,with 15 rats in each of the groups.After the grouping,the rats for experiment received rated intragastric administration with the volume.The administration was done according to such dosages as Traditional Chinese Medicine control group(0.09 g/kg),Western Medicine control group(6.09 mg/kg),shenlijia granular low-dosage group(1.07 g/kg),shenlijia granular middle-dosage group(2.1 g/kg),and shenlijia granular high-dosage group(4.26 g/kg).The rats of the blank group were not given intragastric administration and those of the mode group were intragastrically administered with the same volume of normal saline.The administration frequency and duration were three times each day and 28 days continuously for all the groups.The cardiac function indices of the rats of each group were detected with cardiac color ultrasound,and the cardiac pathology of the rats was determined with HE staining method and was observed with transmission electron microscope.The electrolyte in the urine of the rats was detected with fully automatic biochemical analyser,and the concentrations of serum A VP and urine AQP-2 of the rats of each group were respectively detected with the ELISA kit.Results:1.the general conditions and cardiac functions of the rats of all shenlijia granular dosage groups were improved obviously,the changes in myocardial pathological morphology of the rats were significantly lightened;2.The AVP and AQP-2 levels of all shenlijia granular dosage groups were lowere.With the increase in the dosage of shenlijia granular,the AVP and AQP-2 contents were both reduced gradually while the Ejection Fractions increased.3.24 hours urine volume of rats in each treatment group and the urine ion content,the furosemide group urine volume and urine by ion content is much higher than the blank group,and participation drabanemerosa hebecarpa add each dose group of urine volume and urine of each ion increased significantly,but were lower than the control group.Conclusions:1.shenlijia granular is able to effectively improve the cardiac function of rats with chronic heart failure,raise the left ventricular ejection faction,and reduce the serum AVP and urine AQP-2 contents;2.shenlijia granular is able to effectively treat chronic heart failure,which is related to the fact that it can improve the cardiac function,lower AVP level and down-regulate the AQP-2 expression of the renal V2 receptor and,thereby,increase urine output;3.shenlijia granular,when used to treat chronic heart failure,is able to reduce electrolyte disturbance caused by excessive loss of ions in body fluid. |
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