| Objective:In this study,the software "Traditionnal Chinese Medicine Systerm(V3.0)" was used to explore professor Xie’s diagnosis and treatment ideas for chronic atrophic gastritis and the rule of prescription medication.Through the network pharmacology method,the target and molecular mechanism of the core drug combination of TCM compound in treating this disease by Professor Xie were analyzed from multiple angles,providing preliminary basis and theoretical basis for further experimental research.Methods:The clinical medical records of professor Xie’s treatment of chronic atrophic gastritis in the First Affiliated Hospital of Heilongjiang University of Chinese Medicine from January 2016 to January 2021 were sorted out and standardized treatment was conducted for the screened medical records.The data were input into Excel software to establish a database,and then the quasi-data were imported into Traditionnal Chinese Medicine Systerm(V3.0).A preliminary statistical analysis was conducted on the drug frequency,dosage statistics,meridian return,four qi and five tastes,syndrome type,tongue and pulse symptoms of professor Xie’s prescription for the treatment of the disease.The association rules and cluster analysis between drugs were further extracted and analyzed to obtain professor Xie’s core prescription for the treatment of chronic atrophic gastritis,from which his clinical experience in the treatment of this disease was analyzed,summarized and discussed.With the help of pharmacology analysis platform(TCMSP),the active components of traditional Chinese Medicine and corresponding potential targets of the core drug combination were obtained.The corresponding targets were normalized were unified through UniProt database.chronic atrophic gastritis related targets were obtained from OMIM,GeneCards and DisGeNET databases.Venn diagram was constructed by R3.6.3 software to obtain the aggregate targets related to chronic atrophic gastritis in three disease databases,as well as the common targets of traditional Chinese medicine components--chronic atrophic gastritis.Cytoscape 3.8.0 software was used to establish the "Chinese herbal compound-active component-action target network",STRING database was used to analyze the protein interaction of common targets,and the PPI network diagram was obtained.The PPI network was further analyzed by CytoNCA plug-in to obtain the core targets.Finally,GO and KEGG functional enrichment analysis of differential genes was performed with R3.6.3 software.Results:1.A total of 200 TCM prescriptions were included in this study,including 115 TCM prescriptions,with an average of 20 TCM prescriptions per prescription.The most herbal drugs were warm drugs,followed by cold and peaceful drugs.The most bitter medicine,spicy taste,sweet taste.The main channels of drugs are spleen,lung,stomach and liver.The top 15 drugs in drug frequency statistics included Atractylodes atractylodis,Bupleurum,Citron,Magnolia officinalis,cardamom,Tangerine peel,Aconitum herb,Rhizome,Cuttlebone,Divine Song,Perilla fruit,liquorice root,rubra root,dendrobium,etc.Syndrome classification included liver and stomach qi stagnation syndrome,liver and stomach stasis syndrome and stomach collateral stasis syndrome,with a cumulative frequency of 88%.According to the drug frequency,association rules and comprehensive analysis and repeated confirmations with Professor Xie,the core drug combination(hereinafter referred to as the core prescription)of Professor Xie for the treatment of chronic atrophic gastritis was obtained,consisting of atractylodes atractylodes,Bupleurum,Citron,Magnolia magnolia,cardamom and tangerine peel.2.The key drug combinations were searched in TCMSP,and 50 active components were obtained,and 227 corresponding targets were predicted.chronic atrophic gastritis related targets were retrieved from OMIM,GeneCards and DisGeNET databases,and a total of 784 chronic atrophic gastritis related targets were obtained after collection and duplication removal.R3.6.3 software was used to construct the Venn diagram of the core drug combination-chronic atrophic gastritis target,from which 96 common targets of the core drug combination-chronic atrophic gastritis target were obtained.The main compounds in the core formula were quercetin,nobiletin,beta-sitosterol,dehydrodiisoeugenol and cinetol.PPI network analysis could obtain the first eleven potential core targets of core therapy for chronic atrophic gastritis,including MAPK1,AKT1,MAPK14,IL2,JUN,HIF1A,FOS,HSP90AA1,STAT1,RELA and CTNNB1.GO analysis showed that the biological process of chronic atrophic gastritis gene(BP)was significantly enriched in response to chemical stress,oxidative stress and metal ions.Cell components(CC)are mainly concentrated in the lumen,secretory granule lumen and cytoplasmic sac.Molecular function(MF)is mainly enriched in DNA binding transcription factor binding,ubiquitin like protein ligase binding,antioxidant activity and so on.KEGG enrichment analysis showed that the important signaling pathways related to the treatment of chronic atrophic gastritis by core recipe included IL-17 signaling pathway,TNF signaling pathway,apoptosis pathway,PI3K-Akt signaling pathway,Th17 cell differentiation signaling pathway and HIF-1 signaling pathway.Conclusion:1.Professor Xie holds that the core pathogenesis of chronic atrophic gastritis is based on spleen and stomach weakness,qi stagnation and dampness and heat,accompanied by blood stasis.2.In the treatment of chronic atrophic gastritis,Professor Xie often uses soothing the liver and strengthening the spleen and relieving pain in the stomach as the basic treatment methods.He makes good use of cold and warm products and applies both attack and tonic,and emphasizes "paying attention to the difference between qi and blood,soothing the liver and relieving the stomach".3.Professor Xie’s treatment of chronic atrophic gastritis is characterized by its multi-component,multi-target and systematic regulation,which mainly plays an anti-inflammatory,antioxidant and apoptosis promoting mechanism. |
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