Professor Park Yong-soo’s Medication Law And Network Pharmacology In The Treatment Of Ankylosing Spondylitis

Objectives:1.Use data mining method to explore the medication rules and core drugs of the tutor in the treatment of ankylosing spondylitis,and summarize the clinical experience of the tutor in the treatment of ankylosing spondylitis.2.Conduct network pharmacology analysis on the core drugs of the tutor in the treatment of ankylosing spondylitis,and explore its possible mechanism of action.Methods:1.Collect the first medical records of patients with ankylosing spondylitis diagnosed and treated by the tutor in the Rheumatology Department of the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine from January 2019 to December 2021.After standardizing the medical record data,use Excel to establish The database,with the help of the traditional Chinese medicine inheritance computing platform(V3.0),conducts frequency analysis,association rule analysis and cluster analysis on medical records,excavates the medication rules and core drugs of the tutor in the treatment of ankylosing spondylitis,and summarizes the clinical experience of the tutor in the treatment of ankylosing spondylitis.proven experience.2.Select the core drugs of the tutor for the treatment of ankylosing spondylitis for network pharmacology research,use the TCMSP database to screen out the active ingredients and protein targets of the drug,and correct them in the Uni Prot database;use the OMIM,Disgenet,Drugbank,Genecards databases Retrieve the gene targets of ankylosing spondylitis,find the intersection targets of disease targets and drug targets through Venn diagram,and obtain the relevant targets of core drugs in the treatment of ankylosing spondylitis;use Cytoscape 3.7.2 software to analyze the drug components and drug targets The points are visualized to construct a "drug-ingredient-target" network,and the pharmaceutical chemical components,diseases and related targets are visualized by Cytoscape 3.7.2 software,and a "drug-disease-target" network is constructed;The target data information was imported into the STRING database to generate a PPI network interaction map;R language software was used to perform GO and KEGG enrichment analysis on the intersection targets.Results:1.A total of 136 patients with ankylosing spondylitis were included in this study,including 121 males,accounting for 88.97% of the total cases,and15 females,accounting for 11.03% of the total cases,with a male to female ratioof about 8:1.There were 110 cases in the age range of 20-39 years old,accounting for 80.88% of the total number of patients.2.A total of 6 kinds of tongue images were involved in this study,among which 3 kinds of tongue images appeared.The most frequent occurrence was 108 times of pale tongue,the rest were 25 times of pale and fat tongue,3 times of pale red tongue;3 kinds of tongue coating.,the most frequent occurrence is white fur85 times,the rest are thin white fur 39 times,and greasy fur 12 times;there are 5pulse patterns involved,the most frequent is 86 times of deep and weak pulse,and the rest are tight and stringy pulse.31 times,thepulse was deep and thin for 8times,the pulse was astringent for 6 times,andthe pulse was deep and late for 5times.3.The most frequent occurrences of the four qi of the drug are warm 1148 times,followed by flat 350 times,hot 149 times,cold 122 times,and cool35 times;the most frequent occurrence of the five flavors is sweet medicine 929 times,followed by pungent flavors 883 medicines,807 bitter medicines,77 salty medicines,and 70 sour medicines;the most frequent occurrences of meridians are the kidney meridian 1379 times(33.32%),the liver meridian 1135 times(27.42%),and the spleen meridian 601 times(14.52%);the efficacy categories with the most frequent occurrences were 951 times(52.72%)of tonic type,300 times(16.63%)of expelling rheumatism,and 161 times(8.92%)of promoting blood circulation and removing blood stasis.4.Among the 136 TCM prescriptions included,a total of 108 TCMs wereused,and there were 25 drugs with a frequency of use greater or equal to 20 times.The drugs were sorted from high to low by the frequency of drug use.The top ten drugs were: Morinda citrifolia Tian,Curcuma,Epimedium,Rehmannia glutinosa,Eucommia,Mistletoe,Achyranthes,Fenugreek,Psoraleae,Duhuo.5.Drug association analysis obtained 47 drug patterns,59 association rules,and 8 core drugs,namely Morinda officinalis,Curcuma,Epimedium,Rehmannia glutinosa,Eucommia,Mistletoe,Achyranthes sichuanensis,and Fenugreek.6.Drug cluster analysis obtained 6 groups of drug core combinations: Core combination 1: Morinda officinalis,Curcuma,Epimedium,Fenugreek,Dodder,Chinese yam,Cornus,Rehmannia glutinosa;Core combination 2: Morinda officinalis,Xian Cogongrass,Epimedium,Rehmannia glutinosa,Mistletoe,Eucommia,Achyranthes sichuanensis,Licorice;Core Combination 3: Morinda officinalis,Curcuma,Epimedium,Fenugreek,Eucommia,Achyranthes sichuanensis,Rehmannia glutinosa,Mistletoe;Core Combination 4: Morinda officinalis,Curcuma,Epimedium,Rehmannia glutinosa,Mistletoe,Eucommia,Fenugreek,Antler Cream;Core Combination 5: Curcuma,Epimedium,Morinda officinalis,Psoralea,Rehmannia glutinosa,Eucommia ulmoides,tangerine peel,Poria;core combination 6:Curcuma,Epimedium,Morinda officinalis,Rehmannia glutinosa,Achyranthes sichuan,Duhuo,Mistletoe,Eucommia.7.A total of 67 effective chemical components,293 drug targets,and 1123 disease targets were obtained from the network pharmacology analysis,and 102 targets related to the treatment of ankylosing spondylitis were obtained by the core drug,and the key chemical component of the drug was quercetin.aldol,kaempferol,luteolin,formononetin,beta-sitosterol,isorhamnetin,8-(3-methylbut-2-enyl)-2-phenyl-chromone,icariin;core target The main points are ALB,TNF,IL-6,IL-1β,VEGFA,etc.;GO functional enrichment analysis mainly involves the negative regulation of apoptosis process,cytokine-mediated signaling pathway,and negative regulation of RNA polymerase II promoter transcription KEGGpathway enrichment analysis mainly involves IL-17 signaling pathway,AGE-RAGE signaling pathway,TNF signaling pathway,PI3K/AKT signaling pathway and other signaling pathways.Conclusions:1.Among the 136 patients with ankylosing spondylitis in this study,malewere the predominant;the tongue quality was pale tongue the most frequently,the tongue coating was white fur the most frequently,and the pulse was the most frequent was a weak pulse.2.The four qi used by the instructor in the treatment of ankylosing spondylitis are mainly warm and flat;the five flavors are mainly sweet,acrid and bitter;the main meridians are kidney,liver and spleen;the efficacy categories are tonic,Dispelling rheumatism,promoting blood circulation and removing bloodstasis.3.The core medicines used by the tutor in the treatment of ankylosing spondylitis are Morinda officinalis,Curcuma,Epimedium,Rehmannia glutinosa,Eucommia,Mistletoe,Achyranthes sichuanensis,and Fenugreek.4.The core drug has the characteristics of multi-component,multi-target and multi-channel treatment of ankylosing spondylitis,and its mechanism of action may be by regulating IL-17 signaling pathway,AGE-RAGE signaling pathway,TNF signaling pathway,PI3K/AKT signaling pathway It inhibits the secretion of inflammatory factors,and also affects the expression of IL-6,IL-1β,TNF-α,VEGFA and other related proteins.