Clinical Observation And Analysis Of Clams In The Treatment Of Pertussis Syndrome(Liver Fire Offender Lung Evidence)

ObjectiveThe purpose of this article is to observe the clinical efficacy of Qinghaofang in treating pertussis syndrome(liver fire attacking the lungs)and explore the pharmacological mechanism of Qinghaofang based on network pharmacology,providing ideas for the treatment of pertussis syndrome with traditional Chinese medicine.Methods1.Children with pertussis syndrome who met the inclusion criteria,voluntarily enrolled and signed informed consent were divided into control and treatment groups according to the random number table method.Control group:on the basis of conventional supportive treatment,Lusuge Pill were treated;Treatment group:on the basis of conventional supportive treatment,the traditional Chinese medicine Qinghafang is used for treatment.According to the grading and quantification standards of TCM syndrome,symptom scores of patients in both groups are evaluated,and the score changes of patients in both groups after one week of treatment are statistically analyzed.2.Exploring the potential pharmacological mechanism of Qinghafang in the treatment of pertussis syndrome using network pharmacology.Results1.Clinical study observation:General information comparison:According to the chi-square test,P>0.05,the difference is not statistically significant,indicating that the treatment group and the control group are comparable in terms of gender,age,course of disease,and laboratory results.Comparison of therapeutic effects:The effective rate of the treatment group is 96.97%,while the effective rate of the control group is 88.24%.The therapeutic effect of the treatment group is better than that of the control group.Comparison of total symptom scores:Based on t-test,P<0.05,the difference is statistically significant,indicating that the treatment group performed better than the control group in reducing the total symptom scores.Comparison of major symptom scores:Based on t-test,P<0.05,the difference is statistically significant,indicating that the treatment group performed better than the control group in improving coughing frequency and coughing accompanied by vomiting.In terms of improving the number of single coughs,according to t-test,P>0.05,the difference is not statistically significant,and there is no significant difference between the treatment group and the control group.In comparison of secondary symptom scores:in improving wheezing,night coughing,and chest tightness and shortness of breath,according to t-test,P<0.05,the difference is statistically significant,and the treatment group is better than the control group.In terms of improving facial flushing,coughing up blood,tongue coating,and pulse condition,t-tests showed that P>0.05,indicating that there was no statistically significant difference between the treatment group and the control group.2.Through network pharmacology exploration,it is known that the effective ingredients of the clam formula,such as β-sitosterol,glycyrrhetinic acid B,flavonoids,etc.,act on the disease targets of pertussis syndrome,such as SRC,STAT3,PIK3R1,MAPK1,and act on AGE-RAGE,PD-L1 and PD-1,HIF-1 and other related signaling pathways,participate in the disease process and regulate biological functions,playing a role in anti-inflammatory,anti-oxidative stress,cell apoptosis,immune regulation,etc.ConclusionAfter clinical observation and research,it can be known that Qinghafang significantly improves the frequency of cough attacks,cough with vomiting,phlegm in the throat,night cough and chest tightness and wheezing of pertussis syndrome(liver fire offender lung evidence),effectively reducing the total score of symptoms,and the effect of Qinghafang is comparable to that of Lusuge Pill;Based on network pharmacology validation,the Chinese herbal medicines contained in Qinghafang contain multiple effective ingredients,which participate in the inflammatory process and cell metabolism of whooping cough syndrome through multiple targets and pathways,and regulate the immune environment,playing a role in anti-inflammation,protecting airway mucosal tissue,and accelerating the discharge of inflammatory substances.