Highly Biocompatible Nano-treatment Platform Coated With Indocyanine Green And Polyamine For Enhanced Photothermal Treatment Of Cervical Cancer

Objective: The photothermal material Indo Cyanine Green(ICG)was encapsulated and modified with polydopamine(PDA)and methoxy polyethylene glycol(MPEG)to form ICG@PDA@PEG NPs,to explore its photothermal properties in vitro and in vivo,the photothermal ablation effect of cervical cancer cells(He La cells),and evaluate the photothermal treatment effect of cervical cancer tumor-bearing mice.To provide experimental basis for the conversion of photothermal therapy from preclinical research to clinical research.Methods: 1)First,ICG@PDA@PEG NPs were prepared by "coprecipitation" method,and characterized by UV absorption spectrometer,scanning electron microscope,etc.to detect the absorption spectrum,particle size,etc.of nano-materials;2)Next,use the thermal imager to monitor and evaluate the photothermal properties of ICG@PDA@PEG NPs after 808 nm laser irradiation;3)Then the phototoxicity and dark toxicity of ICG@PDA@PEG NPs on He La cells were analyzed by CCK-8 detection method and flow cytometry.The uptake of ICG@PDA@PEG NPs by He La cells and the ablation effect of ICG@PDA@PEG NPs on He La cells after 808 nm laser irradiation were observed by laser confocal fluorescence imaging system;4)Finally,evaluated the photothermal treatment effect,metabolism distribution and biological safety of ICG@PDA@PEG NPs on cervical cancer bearing mice.Results: 1)ICG@PDA@PEG NPs were successfully prepared,about 140 nm in size,and spherical under scanning electron microscope;2)Compared with ICG,the photothermal properties and photothermal stability of ICG@PDA@PEG NPs modified by PDA and MPEG have been greatly improved;3)The prepared ICG@PDA@PEG NPs could be absorbed by He La cells and produce fluorescence in cells.After co-incubation of ICG@PDA@PEG NPs with He La cells,when there was no laser irradiation,its cytotoxicity was low and it had good biocompatibility.However,after 808 nm laser irradiation,He La cells could appear a large number of apoptosis;4)ICG@PDA@PEG NPs showed good photo-thermal treatment effect on cervical cancer bearing mice,effectively controlled tumor growth,and no obvious damage was found to important organs in the body.Conclusion: In this study,ICG@PDA@PEG NPs prepared by "coprecipitation" showed strong photothermal therapy potential and biocompatibility,and showed good biological safety in vitro and in vivo.ICG@PDA@PEG NPs could be absorbed by tumor cells and could be used for near-infrared fluorescence imaging of cervical cancer.Under the irradiation of 808 nm laser,cervical cancer cells could be ablated,showed a strong killing power of tumor cells.In addition,the evaluation of photo-thermal therapy for cervical cancer bearing mice also showed excellent results,providing a good basis for the follow-up study of photo-thermal therapy for cervical cancer,with a strong future development prospect.