Protective Mechanism Of Shenqi Fuzheng Injection On Kidney Injury In Gastric Carcinoma Bearing Mice

Objective:Explored the protective mechanism of Shenqi Fuzheng injection on kidney injury in MKN45 gastric carcinoma mice by observing the effects of Shenqi Fuzheng Injection on body weight,renal histological morphology and oxidative stress.Methods:1.40 BALB/c-nu mice successfully modeled by MKN45 cells were randomly divided into model group,cisplatin group,Shenqi Fuzheng injection group,Shenqi Fuzheng injection combined with cisplatin group,and 10 normal BALB/c-nu mice were used as blank control group,with 10 mice in each group.2.The body weight was measured every two days,the dosage was adjusted,and the intervention was administered at a fixed time period every day for 10 days.Each group was administered by intraperitoneal injection at a dose of 0.1 m L/10 g.The blank and model groups were injected with normal saline,the cisplatin group and combined group were injected with a single dose of cisplatin（15 mg/kg）on the 4th day of administration,the Shenqi Fuzheng injection group and the combination group were intraperitoneally injected with Shenqi Fuzheng injection（2.73 g/kg）for 10 consecutive days.3.At the last administration,fasting without water was performed for 24 hours.All mice urine was collected and supernatant was taken by centrifugation at low temperature;Eyeball blood was collected from mice,and bilateral kidney tissues were stripped and weighed to calculate kidney index.The kidney tissue was fixed with paraformaldehyde,stained with HE,and the histopathological changes were observed.4.Serum CRE was determined by sarcosine oxidase method,and BUN content was determined by urease method.ELISA method to detect KIM-1,β₂-MG,NAG,NGAL,PA,u ALB level of mouse urine,;The contents of GSH,MDA and SOD activity in mouse kidney tissue were determined by colorimetry.5.The relative expression levels of Nrf2,HO-1 and NQO1 in kidney tissues were detected by Western Blot.6.The Mrna expressions of Nrf2 and HO-1 in kidney tissues were detected by q RT-PCR.Results:1.Dosing weight is no difference between a tumor-burdened rat gastric cancer before each group（P（29）0.05）,each a tumor-burdened rat gastric cancer weight is lower than the blank group（P（27）0.05）;After the treatment,cisplatin group and combined group a tumor-burdened rat weight compared with model group were lower（P（27）0.05）.2.No difference between groups of left kidney index in mice（P（29）0.05）,each a tumor-burdened rat right kidney index compared with the blank group have differences（P（27）0.05）;Shenqi Fuzheng injection can alleviate the kidney pathological changes caused by cisplatin.3.Compared with the blank group and model group,cisplatin group mice were significantly elevated serum levels of BUN and CRE（P（27）0.05）,the joint group of BUN and CRE level decreased significantly in the cisplatin group（P（27）0.05）.Compared with model group,cisplatin group of GSH content in kidney tissue and SOD vitality in mice significantly reduced（P（27）0.05）,the joint group of GSH content and SOD activity significantly increased（P（27）0.05）.Factors compared with model group,ginseng and astragalus injection group of renal MDA level decreased significantly（P（27）0.05）,joint MDA level is cisplatin group reduced.4.The ELISA detection results show that the cisplatin group urineβ₂-MG,KIM-1,NAG,NGAL,PA level increased significantly in the blank group（P（27）0.05）;Factors compared with model group,ginseng and astragalus injection group urineβ₂-MG,KIM-1,NAG,NGAL,PA level significantly decreased（P（27）0.05）.5.Western Blot results showed that the renal tissue Nrf2,HO-1 and NQO1 protein expression of cisplatin group significantly decreased（P（27）0.05）,the joint group compared with cisplatin Nrf2,HO-1 protein expression increased significantly（P（27）0.05）.6.q RT-PCR results show that:compared with model group,cisplatin group kidney Nrf2,HO-1 m RNA relative expression decreased（P（27）0.05）,the joint group of Nrf2,HO-1 m RNA relative to express the cisplatin group increased obviously（P（27）0.05）.Conclusions:Shenqi Fuzheng injection can improve renal function injury and histopathological changes in gastric cancer mice treated by cisplatin,and may exert its antioxidant effect through Nrf2 pathway.