| Objective:To study the antioxidant protective effects of different low-dose of insulin glargine on organs of burned rats with delayed resuscitation.Methods:40 male Sprague-Dawley（SD）rats were randomly divided into five groups,thepseudo-burn group,the group of delayed resuscitation,and insulin glargine 0.5 U,1.0U,and 2.0 U groups,with 8 rats in each group.The rats were immersed in hot water（95.0±0.5）℃for 15 s to establish the third-degree scald model with 30%total surface area.The rats in the sham group were immersed in a 37℃water bath for 15 s tosimulate burns.Insulin glargine(0.5,1.0,2.0 U·kg-1·d-1)was injected subcutaneously into each treatment group 2 h after injury,and normal saline of the same amount（40m L/kg）was injected intraperitoneally into the delayed resuscitation group,andintraperitoneal injection of normal saline 40 m L/kg simulated delay resuscitation 6hours after injury.Abdominal aortic blood samples,heart and kidney tissue were collected immediately after simulating burn in the pseudo-burn group,and 24 hours after burn in other four burn groups.The blood glucose,myocardial enzymes[lactate dehydrogenase（LDH）,creatine kinase（CK）,α-hydroxybutyrate dehydrogenase（α-HBDH）,and aspartate aminotransferase（AST）]and renal function indexes[blood urea nitrogen（BUN）and serum creatinine（SCr）]were measured byspectrophotometry,and the creatine kinase isoenzyme（CK-MB）level was determined by immunosuppression method.The aim was to evaluate the effects of differentlow-dose insulin glargine intervention on blood glucose,cardiac and renal functions in scalded rats with delayed resuscitation.The oxidative and antioxidant indices[xanthine oxidase（XOD）,myeloperoxidase（MPO）,copper-zinc superoxidedismutase（Cu Zn-SOD）,catalase（CAT）,glutathione peroxidase（GSH-Px）,and total antioxidant capacity（T-AOC）]of the heart and kidney tissues of rats were detected by spectrophotometry to analyze the antioxidant effects of different low-dose insulinglargine interventions.Results:Compared with the sham injury group,the blood glucose of the rats in the delayed resuscitation group was significantly increased,the heart and kidney functions were significantly reduced,the oxidation capacity was enhanced,and the antioxidantindicators were significantly reduced.After the intervention with different low doses of insulin glargine,the blood glucose of rats in each treatment group was significantly lower than that in the delayed resuscitation group,the myocardial enzymes and renal function indicators were decreased,the oxidative capacity was decreased,and theantioxidant indicators were increased,and with the increase of the dose of insulin glargine,The decreasing trend of BG,LDH,CK,CK-MB,α-HBDH,AST,BUN,Cr,XOD and MPO of rats in each treatment group gradually increased,while Cu Zn-SOD,GSH-Px,CAT and T-AOC continued to increase.When the dose was 2.0 U·kg-1·d-1,the difference was statistically significant compared with the delayed resuscitation group.[Blood Glucose（mmol/L）:5.91±0.25 vs.11.76±0.36,LDH（U/L）:3332.12±51.6 vs.5008.94±490.12,CK（k U/L）:0.49±0.03 vs.0.85±0.04,CK-MB（U/L）:125.40±12.19 vs.267.52±11.63,α-HBDH（U/L）:122.99±5.37 vs.240.85±13.99,AST（U/L）:11.95±1.81 vs.17.87±1.57,BUN（mmol/L）:4.72±0.15 vs.7.16±0.34,SCr（μmol/L）:87.11±6.51 vs.137.50±11.36,XOD（U/g）:166.29±3.27 vs.204.90±4.82 for heart,63.51±1.46 vs.79.69±1.75 for kidney,MPO（U/g）:1.05±0.02 vs.1.55±0.06 for heart,1.04±0.04 vs.1.87±0.01 for kidney,Cu Zn-SOD（k U/g）:82.95±2.69 vs.56.52±2.26 for heart,94.50±2.73 vs.62.02±1.66for kidney,CAT（U/g）:36.07±2.01 vs.15.15±2.22 for heart,184.49±4.53 vs.156.02±3.96 for kidney,GSH-Px（k U/g）:231.93±8.03 vs.179.48±3.15 for heart,239.63±7.30 vs.172.20±2.09 for kidney,T-AOC（k U/g）:4.85±0.23 vs.2.71±0.11for heart,5.51±0.08 vs.3.50±0.07 for kidney,all P<0.05].Conclusion:Different low-dose of insulin glargine interventions(≤2.0 U·kg-1·d-1)exertedantioxidant protection on the heart and kidney of rats with delayed resuscitation after burns,in a dose-dependent manner. |
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