Effects Of Ketogenic Diet Exposure On Body Weight,liver Lipid Metabolism And Exercise Capacity In Obese Mice

Background: The overweight and the obesity in the world is up to 1 billion,accounting for more than one tenth of the world’s population,and is still growing,so it is urgent to solve the obesity problem.In addition,the effect of the ketogenic diet on the exercise capacity of obese individuals may be significant.The results of many scholars on the effects of the ketogenic diet on hepatic lipid metabolism and exercise performance are inconsistent,and there are few studies on its effects on the exercise performance of obese individuals,so further studies are needed.Objective:In this study,obese mice induced by high-fat diet were used as the study subjects.Under the exposure of ketogenic diet,the effects of ketogenic diet intervention on body weight,exercise capacity,energy metabolism and liver lipid metabolism of obese mice were investigated by recording the changes of body weight and food intake,measuring the one-time exhaustion and grasping force,and analyzing the indexes related to blood lipids,energy metabolism and liver lipid metabolism to provide a theoretical basis for the application of ketogenic diet to obese individuals.Methods: First,6-8 week-old C57BL/6J mice were randomly divided into a high-fat diet-fed obesity modeling group and a standard diet-fed control group,and after the obesity model was successfully induced,the obese mice were randomly divided into a ketogenic diet group(KD,n = 18),a standard diet group(SD,n = 18)and a high-fat diet group(HFD,n = 18),and the original standard diet-fed mice served as the control group(CON,n = 18),and the daily food intake and weekly body weight changes were recorded.After 4 weeks of intervention,one-third of the mice in each group were randomly selected for one-time exhaustion and grasping strength tests,one-third of the mice for metabolic monitoring tests,and one-third of the mice for anesthesia and execution,TG,TC,HDL,LDL,TRH,TSH,FT3,FT4,T4,T4,NPY and leptin levels were detected by kits.q PCR was performed to detect the m RNA expression of Cyp7α,Pgc1α,Cpt1α,Acc1,Srebp1,Fasn in mouse liver,and Western Blot was performed to detect the protein expression levels of HSL,ATGL,SCD1,CIDEA in mouse liver.Results:(1)After 8 weeks of high-fat diet feeding,the success rate of mice model was 63.04%.After grouping,the body weight of mice in SD,KD and HFD groups were similar and significantly higher than those in CON group(p < 0.01).(2)Both the ketogenic diet and the standard diet interventions significantly reduced body weight,white fat weight and lipid droplet size in obese mice(p < 0.01).(3)The ketogenic diet intervention significantly improved the one-time exhaustion time and the distance of the running platform in obese individuals(p < 0.05),with a 63% increase in the mean exhaustion time.(4)The results of metabolic cage analysis showed that the ketogenic diet intervention altered the main energy supply substrate and increased basal metabolism in obese mice(p < 0.05).(5)The ketogenic diet intervention increased serum TG(p < 0.01)and TC(p < 0.05)levels in obese mice,and the standard diet intervention decreased serum LDL levels in obese mice(p < 0.05).(6)The ketogenic diet intervention reduced serum NPY levels in obese mice(p < 0.01),and the standard diet intervention increased serum leptin levels in obese mice(p < 0.05).No significant differences in serum levels of TRH,TSH,FT3,FT4,T3,and T4 were observed among the SD,KD,and HFD groups.(7)Ketogenic diet intervention significantly upregulated liver Cpt1α(p < 0.05),Pgc1α(p < 0.01),Cyp7α(p < 0.01)gene expression in obese mice.(8)Ketogenic diet intervention significantly increased liver ATGL(p < 0.05)protein expression in obese mice.Conclusion: The ketogenic diet intervention significantly reduced appetite,body weight,enhanced aerobic exercise capacity,reduced fat accumulation and enhanced lipid metabolism in obese mice,but it is worth noting that the ketogenic diet may cause greater blood lipid stress and have some impact on health.Therefore,obese patients should be treated with caution when using a ketogenic diet for weight loss.