Experimental Study On The Effects Of ZhiXiaoPingChuanFang On Immune Function Of T Lymphocyte

Objective Asthma is a chronic airway allergic inflammation with eosinophilics and lymphocytes infiltration.Among them the activation of T lymphocytes, especially CD/ helper cells (Th2) play the most important role in the inducement and maintenance of asthmatic airway inflammation.It's the prevailing reaction of Th-, cells that result in a course of inflammation changes mediated by IgE.Up to now, dexamethasone (DM) is still the first choice to the treatment of asthma with partial or total used,but its side -effects are obvious.TCM has got many useful theories and experiences to the treatment of asthma, so the therapy of TCM should be paid more. The study is to investigate the effects of zhixiaopingchuanfang on immune function of T lymphocyte and related cytokines in guinea-pigs asthma model, and to study the immune regulating and controlling mechanism of zhixiaopingchuanfang in the treatment of bronchial asthma.Methods The guinea-pigs asthma model was established with ovalbumin challenge method The guinea-pigs were divided into 6 groups randomly:normal control group , asthma group, DM-treated group, small-dose group of TCM,high-dose group of TCM and integrative group The medicines were gived from the next day of the first inducement to the 8th day.1. The pathologic change of the bronchioles and lung tissues were observed under optical and electron microscope.2 The total and differential white blood cells counts of blood and bronchoalveolar lavage fluid (BALF) were carried out.3 CD/. CD/T cells counts were detected with immunocyutochemistry (ICC) in the peripheral blood mononuclear cells (PBMC).4 Apoptosis of T lymphocyte was detected by TdT-mediated dUTP nick and labeling in PBMC5 Expression of IL-4. IFN- v mRNA was detected by in situ hybridization staining Results 1 Significant infiltration of eosinphils and lymphocytes was observed inairways and lung tissues in asthma group, and the total cell counts especially eosinphils and lymphocytes in BALF were significantly increased. No inflammatory changes were observed in airway in normal control group ,there was no remarkable changes in the inflammatory cells in BALF2 The inflammatory cells in blood and BALF in asthma group were significantly increased than in normal group(p<0.01) There was no remarkable difference in inflammatory cells between the small-dose group and the asthma group(p>0.05) The inflammatory cells were significantly decreased in the high-dose group. DM-treated group and the integrative group than in the asthma group(p<0 05 or p<0.01).The effect of the integrative group was better than the high-dose group and DM-treated group(p<0 05)3 The number of CD/ cells (Th2) was significantly increased in asthma group than in the normal group (p<0.05). but there was no remarkable difference in the number of CD/ cells (Thl) between the asthma group and the normal group (p>0 05) .The number of CD/ cells was significantly decreased in the high-dose group. DM-treated group and the integrative group thanin the asthma group(p<0.05), but there was no remarkable difference in CDS+ cells between them(p>0.05) .The effect of the integrative group was better than that of the high-dose group and the DM-treated group(p<0.05).4.The percentages of apoptosis cells in T lymphocytes were significantly decreased in the asthma group than in the normal group(p<0.01) ,but there was no remarkable difference between the small-dose group and the asthma group (p>0.05).The percentages of apoptosis cells in T lymphocytes were significantly increased in the high-dose group > DM-treated group and integrative group(p<0.05).5.The IL-4 mRNA expression was significantly increased in the asthma group than in the normal group(p<0.05), but there was no remarkable difference in the expression of IFN- Y mRNA between them (p>0.05). There was no remarkable difference between the small-dose group and the asthma group (p>0.05).The IL-4mRNA expression were significantly decreased in the high-dose group > DM-treated group and the i...