Studies On The Chemical Constituents Of Astragalus Membranaceus Bge. Var.Mongholicus (Bge.) Hsiao

Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao, one species of Astragalus, which belong to Family Leguminosae, is a perennial herb growing in the northeast, northwest and southwest of China. The roots of A. mongholicus, known as Huangqi in China, is the most important tonic in the Traditional Chinese Medicine to reinforce "qi" (vital egergy), to strengthen the superficial resistance, and to promote the discharge of pus and the growth of new tissue. It has long been used as an anti-perspirant, adiuretic of atonic, and it has been recorded in many Chinese Herb books. However the studies on the chemical constituents, bioactivities and biopharmacy have seldom been reported before. This doctoral dissertation deals with the studies on the chemical constituents, bioactivities and biopharmacy of A. mongholicus.From the roots of Astragalus mongholicus, thirty compounds were isolated by solvent and chromatography methods, the structures of these compounds were identified, which include isoflavonoids, heterocyclic compounds, benzoid compounds, organic acids and so on. Six compounds are new. They are astragaline A, astragline B, astragaline C, astragaline D, astragaline E, astragaline F. Eleven compounds were isolated from Astragalus for the first time and one compound was isolated from this plant for the first time.The activities of antisenile action and antifungal activity of isoflavonoids from A. mongholicus were studied. The result indicated that the isoflavonoids of A. mongholicus have cell multiplication activity. And we also found that the isoflavonoids was not the active constituents for antifungal activity.To optimize the method of separating isoflavonoids, we separated and purified six isoflavonoids from A. mongholicus by using high-speed counter-current chromatography for the first time. And set up three solvent systems which were suitable to separate isoflavonoids. The result indicate that HSCCC is a rapid, convenient method for separating isoflavonoids. The isoflavonoids obtained by using HSCCC were over 95% purity as determined by HPLC analysis.Isoflavone compounds are the main effective constitutes in A. mongholicus. To explore the metabolism regularity in vivo, we studied the metabolic products of calycosin-7-O-B-D-glycoside in vivo. From the urine of rats who had taken the calycosin-7-O-B-D-glycoside, we isolated 4 metabolic products and 3 of them were identified on the basis of the physical and chemical property and the spectral data, which were the products of calycosin-7-O-B-D-glycoside through hydrolysis, demethyl and dehydroxyl reaction. The results provide the basis for the regularity of the transformation of calycosin-7-O-B-D-glycoside in vivo.We consulted in the literature of chemical constitutes and pharmacological activities in and abroad in recent years, overviewed the research result all-around, and, provide the references for the researches on the plants of Astragalus. At the same time, we consulted and arranged the literature of application of HSCCC in the isolation of effective constituents in plants; moreover, we summarized the methods to choose the two-phase systems in HSCCC.