Study On The Chemical Constituents Of Two Medicinal Plants

Helicia nilagirica Beed. belongs to the plant of Proteaceae. Its seeds are used for the treatment of insomnia and neurasthenia by Jingpo in Yunnan province. Helicid and Helicidol were isolated from its seeds. The Helicid was recorded in the Yunnan local standard at the 1987 edition for treatment of neurasthenia, neurasthenic syndrome and neurovascular headache. The chemical study of the, leaves of this plant has not been reported yet in the literature. For further utilization of this plant resource, the systematic studies of the chemical compounds of the leaves was carried out. Twenty-two compounds were isolated by using the media of non-polar macroreticular resin. Sephadex LH-20, ODS and Silica gel in chromatography from its ethanolic and water-soluble extract and structures were identified with the spectra methods. Five are new compounds including two phytosphingosine glycosides: Helicia cerebroside A and B(HN-l,HN-2) and three flavonol glycosides: Helicianeoside A~C (HN-3-HN-5). The bioactive assay showed that flavonol glycosides had weak activity in inhibiting the ADP-induced aggregation of platelets.Schisandra propinqua (Wall.) Baill var. intermidia A.C.Smith belongs to the family Schisandraaceae and is one of variety of Schisandra propinqua (Wall.) Baill. The bark of this plant is used as an ethnodrug for the treatment of promoting blood circulation to remove blood stasis by YiZu in Yunnan Province. The ZhuoQi TongShu capsule is composed of Erigeron breviscapus, S.propinqua (Wall.) Baill var. intermidia, Ginkgo biloba, Panax notoginseng for curing cerebrovascular disease. There are many research reports on the chemical and bioactive studies of Erigeron breviscapus, Ginkgo biloba, Panax notoginseng, only few about S.propinqua (Wall.) Baill var. intermidia. In order to search the principles constituents, the systematic chemical investigation of the barks of S.propinqua (Wall.) Baill var. intermidia was carried out. From ethanolic and water-soluble extract of this plant, thirty-four compounds were isolated by using the media of non-polar macroreticular resin, Sephadex LH-20, ODS and Silica gel in chromatography and structures identified with the spectra methods. Among them, there are three new compounds including two nitro group phenolic glycosides: 6'-O-a-L-arabinofuranosylthalictoside (SPI-1) and 6'-O-β-D-apiofuranosylthalictoside (SPI-2), and another phenolic glycoside: Vanillic acid 4-O-B-allopyranosidc (SPI-3).Part of these compounds was tested to inhibit the ADP-induced aggregation ofplatelets. (+)-Medioresinol (SPI-14) 4'-Methoxy-benzoyloxy (SPI-23) and 2',3,4,4'-Tetrahydroxychalcone (SPI-21) showed weak activity in inhibiting the ADP-induced aggregation of platelets, Vanillic acid (SPI-24) and Protocatechuicacid (SPI-25) showed strong activity. It was showed that 3(R), 4(R) -epoxy -5(R) -hydroxy -1-cyclohexene -1-carbooxylic acid (SPI-10) possesseded weak activity in preventing apoptosis of microvascular endothelial cells.TLC and HPLC analyses of the ZhuoQi玊ongShu capsule showed that compounds SPI-4, SPI-9, SPI-10, SPI-12, SPI-14, SPI-16, SPI-22 and SPI-24 were detectable in the preparetion.