The Studying On Spermatogenesis Deficiency Of Human Being's

Department of Urology, Xijing Hospital, FMMU, Xi'an, Shaanxi 710032A major cause for male infertility is the spermatogenesis deficiency, in which both quality and quantity of the sperm are included. The diagnosis and treatment of spermatogenesis deficiency have attracted significant attentions and wide studies due to few understanding about the mechanisms. Here we made some relative studies about this disease. First the effects of GABA and L-NAME on sperm were investigated in order to evaluate their possible use in the treatment of spermatogenesis deficiency. Then a cDNA microarray which contained both GABAR, eNOS related and some others spermatogenesis related genes such as RBM was constructed and the differential gene expression profiles between aspermia testes and normal subjects were studied by the cDNA microarray hybridization. The expression and significance of RaplA, an outstanding up regulated expression gene in the present hybridization, were studied in 39 azoospermic and 10 normal subjects by in situ hybridization also with an aim to confirm the array hybridization results at the same time. After that a bioinformatics analysis was employed.We found that GABA could induce AR of the infertile sperms, besides the AR, It could also reinforce the capacitating sperms' HAM by enhancing the VSL, VCL, ALH and BCF (P<0.05); L-NAME might reduce the activity of NOS and then reduced the level of NO in the testis, through this way L-NAME could protect the germ cell from apoptosis in the experimental cryptorchid. Both of them might have roles in the treatment of some infertility.Totally 128 differential expressed genes might be related to azoospermia were found by the cDNA microarray hybridization, among them 56 were up regulated genes and 72 were down regulated. Oncogenes and pro-oncogenes, cellular signal and transducin, metabolism, protein translation, synthesis and some other genes with unidentified functions showed superior involvement in the progress of spermatogenesis deficiency. Of particular importance was the first report of the enhanced expression of RaplA in the azoospermic testes and the mRNA expression of RaplA was confirmed to be stronger in spermatogenic cells of azoospermic testes when compared to that of normal fertility by in situ hybridization.In conclusion, screening the differential gene expression profiles between the human normal and azoospermia testes by cDNA microarray can be used in the further investigation of azoospermia related genes. RaplA may play certain roles in the development and progression of azoospermia.