The Study Of Antiosteoporotic Constituents Of Sambucus Williamsii Hance

This thesis focuses on isolation and structure elucidation of bioactive constituents from Sambucus williamsii and the effects of isolated compounds on proliferation and differentiation on the rat osteosarcoma clonal cell line, UMR106.28 Traditional Chinese Medicine species were screened while UMR106 cell proliferation was used as in vitro marker and Sambucus williamsii, which has been used for prevention and treatment of bone diseases since ancient time, was chosen to go on with further investigation. Besides the effects on stimulating UMR106 cell proliferation, the 60 % ethanol extract of this plant also showed osteoprotective effects on ovariectomized rats. Bioactivity-guided isolation procedure afforded 47 compounds. The structures of 46 compounds were elucidated by physical chemical properties and spectroscopic analysis as:1: emodin; 2: betulin; 3: betulinic acid; 4: oleanolic acid; 5: 4-hydroxy-3-methoxybenzal--dehyde (vanillin); 6: 4-hydroxy-3-methoxyphenyl ethyl ketone (acetovanillone); 7: 4-hydroxy--3-methoxyphenyl propenaldehyde (coniferaldehyde); 8: ursolic acid; 9: hexadecanoic acid; 10: (-)-syringaresinol; 11: (-)-pinoresinol; 12: 4-hydroxy-3, 5-dimethoxybenzaldehyde (syringal--dehyde); 13: (-)-erythro-1, 2-bis(4-hydroxy-3-methoxyphenyl)-1, 3-propanediol; 14: (-)-1-(4--hydroxy-3-methoxyphenyl)-2-{4-[(1E)-3-hydroxy-1-propenyl)]-2-methoxyphenoxy}-1, 3-pro--panediol ((-)-guaiacylglycerol-β-O-4'-coniferyl ether); 16: (-)-erythro-1-(4-hydroxy-3--methoxyphenyl)-2-[4-(3-hydroxypropanyl)-2-methoxyphenoxy]-1, 3-propanediol; 17: (-)-threo--1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropanyl)-2-methoxyphenoxy]-1, 3-propane--diol; 18: (-)-2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxy-3-methoxyphenyl)methyl]-3--tetrahydrofuranmethanol ((-)-lariciresinol); 19: (2R, 2S)-2-(4-hydroxy-3-methoxyphenyl)-3--hydroxymethyl-7-methoxy-2, 3-dihydro-5-benzofuranpropanol (dihydrodehydodiconifyl alcohol); 20*: erythro-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(4-hydroxy-3-methoxycinnamoyl--oxy)propanyl-2-hydroxyphenoxy]-1, 3-propanediol; 21*: threo-1-(4-hydroxy-3-methoxy--phenyl)-2-[4-(4-hydroxy-3-methoxycinnamoyloxy)-propanyl-2-hydroxyphenoxy]-1, 3-propane--diol; 22*: (-)-2-{4-[2, 3-dihydro-3-hydroxymethyl-7-hydroxy-5-(4-hydroxy-3-methoxy-...