| Chemical Analysis of Traditional Chinese Medicine Prescription- Sijunzi DecoctionSijunzi decoction is one of the famous traditional Chinese medicine (TCM) prescriptions, consisting of Panax ginseng, Poria cocos, Atractylodes macrocephala and Glycyrrhiza uralensis. It has been either used alone to replenish or invigorate the intestine and stomach function, strengthen the body immunity, or as a complement to the west drugs in treatment of various cancers.To investigate the effectiveness of Sijunzi decoction, characterization of active components in the individual herbs and in the prescription are crucial. Traditional high performance liquid chromatograph was first applied in the quantitative analysis of multi-components in Sijunzi decoction, which provides the valuable criteria for TCM quality control. At the same time the results showed that the major components in the prescription were from Panax Ginseng and Glycyrrhiza Uralensis.To further study of the medicinal decoction, LC-ESI/MS/MS was used to characterize and identify the active constituents in it, which revealed the principle of the herbs intrinsic combination. In summary, twenty-eight components were identified in Sijunzi decoction, in which twelve were from herb Panax Ginseng, and sixteen from herb Glycyrrhiza Uralensis. LC/MS was also applied for the comparison of relative peak intensity of major active components in Sijunzi decoction and the single herb extracts. Obvious differences of concentration ratios of major active components in Sijunzi decoction from single herbs of Panax Ginseng and Glycyrrhiza Uralensis were detected.To further study the metabolites of Sijunzi decoction in rat plasma, incubation of the active ginsenosides in artificial gastric liquid in vitro was prepared. The metabolites after incubation were analyzed by using LC-ESI/MS/MS, which showed that the major metabolites of ginsenosides were the hydrolysis products and their corresponding hydrates.
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