An Analysis Of Involving Factors For Setting Up And Patch-clamp Recording Lab. A Series Of Study For Transient Outward Potassium Current In Human Myocytes.

According to our research objects, financial ability, and developmental plan of the Lab., we established an ion-channel patch-clamp recording system by selecting some instruments and software which are technologically advanced, high quality manufacuring standards, ready-to-use, internationally employed and expert technical support. Our system has been showing little disturbing from noise, vibration, etc. and operating smoothly.An stable method for human myocytes ion-channel patch-clamp recording were established. Detailly, 1. Isolation method of single atrial and ventricular cells from human hearts, two enzymatic steps dissociation method that yields living cells ;2.a series of study for transient outward potassium current in human myocytes, this is first systemically research about human myocytes I_tol in our country. â‘  Comparison of I_tol in young and adult human atrial myocytes. we found evidences for age-related changes in I_tol, including inactivation time course, steady-state inactivation value, recovery kinetics. â‘¡A study of changes of transient outward potassium current (I_tol) in right atrial...