Model Establishment Of Gastric Ulcer Of Water Immersion Stress Combined With Cold And Hot Syndrome And Study Of Cold And Hot Formula On Formula And Syndrome Correlation

Object:To establish evaluation criterion of the models of stress-restrainulcer with cold and hot syndrome.Reveal the essential of stress-restrain ulcermodels with cold and hot syndrome.Observe the mechanism,material basisand regulation of formula-symptom corresponding of Dahuang HuanglianXiexin Decoction and Lizhong Pill on the models of stress-restrain ulcer withcold and hot syndrome.Methods:Use cold factor(cold water,0.3mol/L NaOH)and hot factor(8%pepper powder,60% ethanol mixed solution)to establish the model of coldand hot syndrome combined with water immersion stress method which hasbeen received by domestic and abroad scholar,and treat with DahuangHuanglian Xiexin Decoction of cold property and Lizhong Pill of hot property.1.Killed the rats seven hours after water-stress.Detected the ulcer index,thecontent of TNF-a,IL-8,ET,NO and INOS in blood serum.2.Detected theEGF,TGF-a,SOD and MDA in the stomach tissue.3.Detected the content of5-HT and NE in the brain tissue.Results:1.In ulcer index aspect,compared to the water-stress group,theulcer index of the cold syndrome with water-stress group and the hotsyndrome with water-stress group were increased apparently(P＜0.01)Theindex of the cold syndrome with water-stress group treated with Lizhong Pilland the hot syndrome with water-stress group treated with DahuangHuanglian Xiexin Decoction were decreased apparently compared to the coldsyndrome with water-stress group and the hot syndrome with water-stressgroup respectively(P＜0.01)2.Compared to the blank group,the content ofSOD.EGF and TGF-a of the cold syndrome with water-stress group and thehot syndrome with water-stress group decreased(P＜0.01).The content ofTGF-a decreased in the hot syndrome with water-stress group(P＜0.01)compared to the cold syndrome with water-stress group.The content of thecold syndrome with water-stress group treated with Lizhong Pill and the hotsyndrome with water-stress group treated with Dahuang Huanglian XiexinDecoction were increased apparently compared to the cold syndrome with water-stress group and the hot syndrome with water-stress group respectively(P＜0.01 or P＜0.05)3.Compared to the blank group and the cold syndromewith water-stress group,the contents of MDA.NO.INOS.IL-8.TNF-a andNE increased in the hot syndrome with water-stress group(P＜0.01 or P＜0.05).Compared to the hot syndrome with water-stress group,the contents of MDA.NO.INOS.IL-8.TNF-a and NE decreased in the hot syndrome withwater-stress group treated with Dahuang Huanglian Xiexin Decoction(P＜0.01or P＜0.05).4.Compared to the blank group and the hot syndrome withwater-stress group,the contents of 5-HT and ET increased in the coldsyndrome with water-stress group(P＜0.01 or P＜0.05)After treated byLizhong Pill,compared to the cold syndrome with water-stress group,thecontents of 5-HT and ET decreased in the cold syndrome with water-stressgroup(P＜0.01)Conclusions:1.The mechanism of cold syndrome and hot syndrome with water stresshave relationship with general factor such as nerve-endocrine and local factorsuch as protection function and damage function of gastric mucosa.2.Hot syndrome with water stress ulcer model has relationship with thedecreasing of SOD,EGF and TGF-a,and has relationship with the increasingof MDA,NO,INOS,TNF-a,IL-8 and NE.All these can be seen asmicro-indications for clinical acute ulcer with hot syndrome.3.Cold syndrome with water stress ulcer model has relationship with thedecreasing of SOD,NO,EGF and TGF-a and has relationship with theincreasing of 5-HT and ET.All these can be seen as micro-indications forclinical acute ulcer with cold syndrome.4.The mechanism of the cold formulae,Dahuang Huanglian XiexinDecoction,to prevent the water-stress induced ulcer with hot syndrome hasrelationship with increasing the activity of SOD,decreasing the content ofMDA,reducing the inflammatory reaction of NO.INOS,stimulating thereleasing of EGF,TGF-a,inhibiting the releasing of IL-8 and TNF-a anddecreasing the activity of sympathetic adrenal and content of NE.5 The mechanism of the hot formulae,Lizhong Pill,to prevent the water-stress induced ulcer with cold syndrome has relationship withincreasing the activity of SOD,increasing the content of NO,decreasing thecontent of ET and mproving the gastric mucosal blood flow.stimulating thereleasing of EGF,TGF-a.Increasing the activity of sympathetic adrenal anddegrading content of 5-HT.