In Vitro Assessment Of The Biological Behavior Of The Invasive Pituitary Adenoma And A Preliminary Investigation Of Its Medicine Treatment

Invasive pituitary adenoma bears both the characteristic of the benign tumor and that of the malignant tumor.Despite their benign histological appearance,tbey are known to have a propensity to invade surrounding structures,which not only precludes complete surgical excision but also imposes significant neurological and endocrinological morbidity.There is a higher recurrence rate in invasive pituitary adenomas.A clear need exists for a logical diagnosis strategy and an alternative treatment.Morever,it is a great challenge to keep the pituitary adenoma cell long enough in vitro with stable viability and functions.In this study,19 specimens of pituitary adenomas were gathered,in spite of the function of the tumor and the sex and age of the patient.They were separated into invasive and noninvasive groups according to clinical standard for the invasive pituitary adenoma. In vitro primary cell culture was performed using three different methods. The 15 viable cultures showed different performances related to the way of specimen-taking,the cell culture medium,and the cell culture method. Variability existed among different patients or different cells.The cells showed best viability on day 4-7.The cells then were cultured in the Boyden Chamber system.It came out that the Boyden Chamber system could represent the internal environment well under certain circumstances with the stronger aggressiveness of invasive pituitary adenoma cells than noninvasive pituitary adenoma cells,although weaker than other classical malignancies. 5-Aza-2'-deoxycytidine could somewhat inhibit the aggressiveness,and meanwhile increase the E-cadherin expression.Immunohistochemical technique and reverse transcription-polymerase chain reaction analysis revealed that E-cadherin expression was significantly reduced in invasive pituitary adenoma.5-Aza-2'-deoxycytidine may improve the E-cadherin expression through reversing methylation and reduce cell aggressiveness sequentially.