| Study backgroundFracture is the common disease in the clinical practice. Along with social economy's development, the transportation is highly developed, moreover China enters into the aging society gradually, the fracture becomes more frequently. Statistically, there is approximate 5%-10% fractures may lead to the slow cicatrization and disunion by each kind of reason. At present, there are few research about promoting healing by medicine overseas. The domestic research also stresses on the oral administration of traditional Chinese medicine, the level of research about traditional Chinese medicine external using promoting healing is not high compared to the oral administration. The history is long in Fracture treated with the Chinese medicine, and also has the rich experience. "Occupied Extends Chinese Jan" unearthed in 1931 had recorded the medicine external use which could treat wound by military doctor in Han Dynasty. Obviously it is very common as early as in the Qin and Han Dynasty. Therefore, curative effect and the mechanism of the external use of traditional Chinese medicine through modern molecular biology and cytobiology has the vital practical significance and the social meaning."Guoxin ostalgia ointment" is a folk recipe and comes from Jilin Province. It is a kind of external use medicinal and can promote fracture healing. It has been many years in clinical practice and has the excellent curative effect."Guoxin ostalgia ointment"had cured massive patient with bone fractures, the bone do not link and the osteonecrosis. But the mechanism is not clear yet, through testing biochemical indicator, X ray grading, tissue slice analysis, the collagen typing, immunohistochemistry test of the bone morphogenesis protein (BMP-2) and hybridization in situ test for bone morphogenesis protein (BMP-2), author hopes to reveal the mechanism of promoting fracture healing and also hopes to provide more scientific evidence for external use of traditional Chinese medicine from the view of modern medicine.Study objectiveTo study the therapy effect of "Guoxin ostalgia ointment" and to reveal the possible mechanism of promoting fracture healing.Study methodsFracture models were made by operation.3mm bone defect was made on the middle shafts of both Japanese rabbits in each bone. Observe the post-operation general conditions of the Japanese rabbits. Thirty rabbits were randomly assigned to receive "Guoxin ostalgia ointment" and blank control groups.15 rabbits in each of the two groups. Then each group were assigned to 3 sub-groups. With specimen collection from rabbits postoperative 1 week (A),2 week (B) and 4 week(C).All of the experimental rabbits were made to the bone defect model with 3mm wide to the pulp chamber located on the midpiece of bi-radial. Rabbits in blank control group were received by gypsum exclusively. Rabbits in experimental group were received by"Guoxin ostalgia ointment" external use add gypsum fixation. Experimental group changed addressing per two days. Execute the rabbits in A sub-group after first week. Execute the rabbits in B sub-group after second week. Execute the rabbits in C sub-group after fourth week. Test ALP, Calcium, Phosphorus and Magnesium from blood of ear vein. Shot the bi-radial X ray film. T-test was used to statistic the three group(A,B,C). Pathology test by the method of HE, Masson dyeing. light microscope observation ofâ… ,â…¢collagen bitter taste sirius dyeing. BMP-2 was tested by the method of immunohistochemistry and hybridization in situ. T test was applied to the A,B,C each group after testing the value of MOD.Study resultsEach group of rabbit's general conditions is good. Biochemical indicator of 1,2,4 week after operation showed:There is no difference between experimental group and blank control group on the effect of blood density of ALP, Calcium, Phosphorus, Magnesium and calcium-phosphorus product. The result of postoperative 1st week showed:"Guoxin ostalgia ointment" can enhance the BMP-2 expression and bony callus formulation; Reduce healing time. Callus formation was occurred by the radiological examination and there is no bony callus in the blank control group. The result of postoperative 2nd week showed:There exist bony callus in both two group. Bony callus stuffed the bone defect area and massive external callus formation appeared in the experimental group. And there is no external callus but soft callus stuff in the blank group. The result of postoperative 4th week showed:medicine group's bone cerebral cortex reconstruction was appeared in the damage area, the backbone contour restored, the medulla cavity passed again. And callus filled up basically in the control group damage area, connective callus shadow still existed, the medulla cavity had not pass. Through macroscopic observation of X ray, the store of medicine group is higher than the control group and there is obvious difference in statistics. Through the HE and Masson dyeing, in the 1st week, there appeared bony callus in the medication group and there existed obvious bony callus in the 2nd week. In the 4th week, the collagen reduction was obvious in the defect area and the quality was good; In the control group, callus formed in the 1st week, there did not have the external callus in the 2nd week, there existed massive collagens in the 4th week in the damage area and the requirement quality was worse than the experimental group.â… ,â…¢collagen bitter taste sirius dyeing result:experimental group appeared earlier than the control group and had more I collagen; BMP-2 was tested by the method of immunohistochemistry and hybridization and the result indicated that the experimental group appeared earlier than the control group, the expressive degree was obvious outweigh the control group and there was obvious difference in statistic.Conclusion(1) "Guoxin ostalgia ointment" doesn't affect the ALP, Calcium, Phosphorus, Magnesium and calcium-phosphorus product.(2) "Guoxin ostalgia ointment" can enhance the expression of I type collagen.(3)"Guoxin ostalgia ointment"can regulate the formulation,externalization and promote the BMP-2 expression.(4) "Guoxin ostalgia ointment" can make the bony callus appears earlier, improve the bone healing quality, reduce the healing time and promote the healing progress. |
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