Study On Stress Related Body Function Changes And Its Laboratory Evaluation Methods

The harmonious and balance between human and environment is the basis of human health, but the human healthy condition could be effected by over stress of the various environmental factors. These studies intended to explain that food intolerance, unhealthy life styles(including smoking and drinking), and exercise training induced biological changes, such as the levels of trace elements and the immune function, as well the immune status changes after hyperbaric oxygen therapy. The results and findings of these studies would provide some new clue for the prevention and treatment of stress related injury and disease.In these studies enzyme immunoassay was used to detect14food-specific IgG antibodies in serum. Differential potentiometric stripping method and stripping voltammetry were used to detect the levels of7trace elements in peripheral blood, including cadmium, lead, copper, iron, zinc, calcium, magnesium. Flow cytometry method was used to analyze percentages of T, B, NK cell subpopulations from peripheral blood. The results were as follow.a. Compared with control, there are differences of trace elements levels and percentages of T, B, NK cell subpopulations among each group, in which every participant had a common certain kind of positive food-specific IgG antibody. With egg-specific IgG antibody elevating, it was found that the percentages of CD3+CD4+cells, CD4+/CD8+ratio decreased and the percentages of CD3-CD19+cells increased.b. Compared with control, there were changes of blood trace elements levels and the number of cells in lymphocyte subsets in participants who liked smoking or (and) drinking compared with control.c. Compared with control, there were some changes in blood trace elements levels and immunity function improving in long-term physical training people.In the study, body immunity status in combination therapy with hyperbaric oxygen was analyzed and cases which had hyperbaric oxygen therapy were reviewed. The results were as follow.a. Acutephasereactants expression was depressed after HBO therapy combined with drugs which had anti-superoxide function, in which body immunity increased, inflammatory status released and certain diseases recoverd.b. HBO therapy had a clear curative effect on many diseases.In conclusion,various stress factors may cause the biological changes mainly for the adjustment and regulation of the immune function and the level of the trace element. These stress related environment factors might lead to stress injury, and meanwhile it also could be used as disease prevention and therapy methods.