| The mortality rate of lung cancer is the world’s top ranked malignant tumours, cisplatin (DDP) is a method of treatment afterradiotherapy operation. Chemotherapy caused lung cancer drug resistance is a common problem, in addition to platinum main side effects, such as:renal toxicity and gastrointestinal reaction, limit its clinical application. So the exploration of platinum drug delivery of new method, is expected to become a hot spot in the research of tumor at home and abroad. Many research results show that chemotherapy can reduce the adverse reaction of tumor patients with chemotherapy, improve efficiency and prolongs survival period, is expected to become a new method for clinical chemotherapy.PurPose:A clear non small cell lung cancer using Ziwu Liuzhu method under the guidance of Chrono chemotherapy assisted by the hour to take health traditional Chinese medicine and traditional Chinese medicine (combination therapy reduced referred to time) the effectiveness and side effects of the situation, provide the basis for clinical trials. References to the Vimentin test, the interstitial cell marker factor, its high expression marks the epithelial mesenchymal transition (Epithelial-mesenchymal transition, EMT) process, is also the start of tumor metastasis occurs, thereby to explore whether hour treatment for inhibiting tumor metastasis. Study and the introduction of chronotherapy and regulation of endoplasmic reticulum stress inhibitstumor relationship. Using flow cytometry, Western blot, RNA interference technology, then explains the various mechanisms.Methods:The first part:Experimental study:Via endoplasmic reticulum stress and apoptosis studies when therapeutic effect of chemotherapy for lung cancer mice. Establish regular mice model, the establishment of positive control group, You shi group, Chen Shi Group, tumor bearing group. The experimental group with cisplatin in you Shi and Chen Shi during two time of application, the control group to avoid you Shi, on the two time periods in tumor control group without any drug. Observation of Chrono chemotherapy on tumor weight and inhibition rate of tumor, tumor size effect etc. Detection of apoptotic cells in mice by TUNEL method to detect the expression of Caspase-4, RT-PCR method, to observe the pathological changes of mice.The second part:Clinical research:chemotherapy and drug treatment NSCCL hour through clinical application containing cisplatin in different time, to explore the therapeutic value of the new method of the hour, and seek for the treatment of tumor. Will be incorporated into the observation of 90 cases were divided into groups, when Chen Shi Group, control group according to the time of drug laws. Determination of the indexes of renal function, the reaction of digestive tract, digestive tract reaction index comparison, average the vomiting times comparison, determination, general quali ty of life after the treatment of lung cancer, tongue distribution before and after treatment of TCM syndrome curative effect comparison, the two groups before and after treatment of TCM syndrome integral contrast, blood routine examination, B2- urine microglobulin average number of indicators and etc. when the treatment effect is obtained.Results:The first part:1. Tumor weight and inhibition rate of tumor:the determination results of experiment group tumor weight and the tumor inhibition rate and tumor results compared to the control group, the treatment groups were lower than the average tumor weight of tumor bearing control group, the difference was statistically significant (P<0.01); There was a significant difference between the treatment group and the treatment group the hour (P<0.05) hour treatment groups, no significant difference (P>0.05).2.results of TURNEL apoptosis rate:In the treatment group and the tumor as compared to the control group, treatment group were higher than the rate of apoptosis in tumor control group (9.03+1.53), the difference was statistically significant (P<0.01). The treatment group were compared between treatment group, cisplatin apoptosis rate (20.64+2.76) higher than the treatment group tumor (9.03+1.53) other also so, Chen Shi Group (33.92+2.17) higher than when the treatment group (30.08+3.06), Chen Shi Group and the treatment group was higher than that when the rate of apoptosis of cisplatin treatment group differences are statistical significance (P<0.05), and Chen Shi treatment group and treatment group differences were small when, no statistical significance (P> 0.05).3. Caspase-4 expression was detected by RT-PCR method:Under light microscopy, localization of caspase-4 protein in lung adenocarcinoma cell cytoplasmic region, the morphology of the cells were round or oval, expression was seen in each group, control group with tumor protein expression is weak, brown yellow pale staining; the expression of caspase-4 protein was significantly enhanced compared with blank control group, the treatment group effect, brown-Tan expression.4.Histopathological observation results:Tumor control group: tumor cells arranged in dense, tumor tissue necrosis area of rare or small necrotic areas; tumor cells with marked atypia, nuclear hyperchromatic interstitial rare inflammatory cell infiltration. Cisplatin group:tumor cell density higher than that in tumor control group decreased, tumor tissue was seen of necrosis, tumor cell swelling, interstitial inflammatory cell infiltration. Chen Shi treatment group:tumor cell density higher than that in tumor control group decreased, tumor tissue was seen necrosis; tumor cell swelling; interstitial inflammatory cell infiltration. Yinshi treatment group:tumor cell density higher than that in tumor control group decreased; tumor tissue in scattered necrosis; tumor cell swelling; interstitial inflammatory cell infiltration.The second part:1. On the application of cisplatin in NSCCL patients during chen shi group, gastrointestinal reaction and other indexing the same period, but the digestive tract reaction duration was significantly shortened, so that the average reduction in the number of patients with vomiting, recovery of patients with gastrointestinal function to chemotherapy.2. When application of cisplatin in NSCCL patients during You shi, impairment of renal function lower than other hour;3. Cisplatin application specific hour segment selection, should be based on individual patients were selected when, Chen Shi or application, does not recommend the other hour cisplatin. |
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